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Mouse CYP17 / CYP17A1 ELISA Kit (Sandwich ELISA) - LS-F25343 Best Seller
Catalog
Size
Price
LS-F25343-1
1 plate
$810
Available Discounts
10% off 5+ units

Product Description

LS-F25343 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse CYP17 / CYP17A1 in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CYP17 / CYP17A1 as low as 1.56 nanograms per millilter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CYP17 / CYP17A1
Synonyms
CYP17A1, CYP17, CYPXVII, CPT7, Cytochrome P450-C17, Cytochrome P450c17, p450C17, Steroid 17-alpha-monooxygenase, Cytochrome P450 17A1, Cytochrome p450 XVIIA1, S17AH
Reactivity
Mouse
Manual
Intended Sample Types
Cell Lysates, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
1.56 - 100 ng/ml
Sensitivity
1.56 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Sample Diluent
  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Wash Buffer (30x concentrate)
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Restrictions
For research use only.
About CYP17 / CYP17A1
CYP17 / CYP17A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. P05093 NM_000102 NP_000093.1


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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 10/19/2018

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