Competitive EIA (enzyme immunoassay) kit
1,25 Dihydroxy Vitamin D3
This assay has high sensitivity and excellent specificity for detection of mouse DHVD 3. No significant cross-reactivity or interference between mouse DHVD 3 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse DHVD 3 and all the analogs, therefore, cross reaction may still exist.
Intended Sample Types
Colorimetric - 450nm (TMB)
1000 - 5000 fmol/l
Intra-assay: CV%<10% Inter-assay: CV%<15%
Short term: 4°C; Long term: see manual.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Stop Solution
- Adhesive Plate Sealers
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated DHVD 3 Hapten. The competitive inhibition reaction is launched between with HRP-conjugated DHVD 3 Hapten and DHVD 3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of DHVD 3 in the sample. The color development is stopped and the intensity of the color is measured.
For research use only.