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Mouse 1,25 Dihydroxy Vitamin D3 ELISA Kit (Competitive EIA) - LS-F10143

Best Seller
Catalog
Size
Price
LS-F10143-1
1 plate
$640
Available Discounts
10% off 5+ units

Product Description

LS-F10143 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse 1,25 Dihydroxy Vitamin D3 in samples of Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of 1,25 Dihydroxy Vitamin D3 as low as 250 femtomoles per liter.

Specifications

Type
Competitive EIA (enzyme immunoassay) kit
Target
1,25 Dihydroxy Vitamin D3
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse DHVD 3. No significant cross-reactivity or interference between mouse DHVD 3 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse DHVD 3 and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Serum
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
1000 - 5000 fmol/l
Sensitivity
250 fmol/l
Precision
Intra-assay: CV%<10% Inter-assay: CV%<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • User Manual
  • 96-well Microplate
  • Detection Reagents
  • Wash Buffer
  • Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Background
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated DHVD 3 Hapten. The competitive inhibition reaction is launched between with HRP-conjugated DHVD 3 Hapten and DHVD 3 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of DHVD 3 in the sample. The color development is stopped and the intensity of the color is measured.
Restrictions
For research use only.

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Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: United States
Date Requested: 4/19/2019
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