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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human TNFRSF10A / DR4 ELISA Kit (Custom ELISA) - LS-F365

Best Seller
Catalog
Size
Price
LS-F365-1
1 plate
$700
Available Discounts
10% off 2-4 units
20% off 5+ units

Product Description

LS-F365 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human TNFRSF10A / DR4. It is based upon a Custom assay principle.

Specifications

Type
Custom ELISA kit

All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.

Target
TNFRSF10A / DR4
Reactivity
Human
Specificity
The antibody pair provided in this kit recognizes human TNFRSF10A / DR4.
Manual
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
To be determined
Sensitivity
To be determined
Storage
Short term: 4°C; Long term: see manual.
Kit Components
Typical components include:
  • Microplate - Item A
  • Wash Buffer Concentrate (20x) - Item B
  • Standards - Item C
  • Assay Diluent A - Item D
  • Assay Diluent B - Item E
  • Biotinylated Detection Antibody
  • HRP-Streptavidin concentrate - Item G
  • TMB Substrate
  • Stop Solution - Item I
  • Adhesive Plate Sealers
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl standard or sample to each well.
  3. Incubate 2.5 hours at room temperature or overnight at 4°C.
  4. Add 100 µl prepared Detection Antibody to each well.
  5. Incubate 1 hour at room temperature.
  6. Add 100 µl prepared Streptavidin solution.
  7. Incubate 45 minutes at room temperature.
  8. Add 100 µl TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 minutes at room temperature.
  10. Add 50 µl Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions
For research use only.
About TNFRSF10A / DR4
NM_003844

Popular TNFRSF10A / DR4 Elisa Kits

Reactivity: Mouse
Range: 0.156-10 ng/ml
Reactivity: Human
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Reactivity: Human
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Reactivity: Human
Range: 0.312-20 ng/ml

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Requested From: United States
Date Requested: 7/15/2019
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