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Human TIMP4 ELISA Kit (Sandwich ELISA) - LS-F9990


Size Price
1 plate $570
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)

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100% Guaranteed 100% Guaranteed
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
This assay has high sensitivity and excellent specificity for detection of human TIMP-4. No significant cross-reactivity or interference between human TIMP-4 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human TIMP-4 and all the analogs, therefore, cross reaction may still exist.
LS-F9990 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human TIMP4 in samples of Human Milk, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of TIMP4 as low as 0.005 nanograms per millilter.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Human Milk, Plasma, Serum, Tissue Homogenates
50 - 100 µl
96-Well Microplate
0.313 - 20 ng/ml
0.005 ng/ml
Intra-assay: CV%<8% Inter-assay: CV%<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping. ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Assay plate (12 x 8 coated Microwells)
  • Standard (Lyophilized)
  • Biotin-antibody (100 x concentrate)
  • HRP-avidin (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin Diluent
  • Sample Diluent
  • Wash Buffer (25 x concentrate)
  • TMB Substrate
  • Stop Solution
  • Adhesive Strip (For 96 wells)
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
2°C to 8°C
For research use only.

About TIMP4

Q99727 NM_003256 NP_003247.1

TIMP4 Elisa Kit, Metalloproteinase inhibitor 4 Elisa Kit, TIMP-4 Elisa Kit

TIMP4 belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Requested From: United States
Date Requested: 10/27/2016

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