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Human S1PR1 / EDG1 / S1P1 ELISA Kit (Competitive EIA) - LS-F39322

Catalog Size Price
LS-F39322-1 1 plate Unavailable

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LS-F39322
Competitive EIA (enzyme immunoassay) kit
S1PR1 / EDG1 / S1P1
Human
LS-F39322 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human S1PR1 / EDG1 / S1P1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of S1PR1 / EDG1 / S1P1 as low as 0.094 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.156 - 10 ng/ml
0.094
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP Streptavidin Conjugate Diluent
  • Sample Diluent
  • Standard (Lyophilized)
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Adhesive Plate Sealers
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with S1PR1. During the reaction, S1PR1 in the sample or standard competes with a fixed amount of S1PR1 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to S1PR1. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of S1PR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Short term: 4°C; Long term: see manual.
For research use only.

About S1PR1 / EDG1 / S1P1

P21453 NM_001400 NP_001391.2

S1PR1 ElisaKit, CD363 ElisaKit, CD363 antigen ElisaKit, CHEDG1 ElisaKit, ECGF1 ElisaKit, EDG-1 ElisaKit, D1S3362 ElisaKit, EDG1 ElisaKit, S1P1 ElisaKit, S1P receptor Edg-1 ElisaKit, S1P receptor 1 ElisaKit

G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1.

 

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: 
Date Requested: 5/26/2018

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