Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
For the detection and quantification of endogenous levels of natural and/or recombinant Human SPARC proteins.
LS-F24666 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Osteonectin / SPARC in samples of Cell Culture Supernatants and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Osteonectin / SPARC as low as 20 picograms per milliliter.
Cell Culture Supernatants, Serum
96-Well Strip Plate
Colorimetric - 450nm (TMB)
780 - 50000 pg/ml
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Coated 96-well Strip Plate
Biotinylated Detection Antibody (100x)
Avidin-Biotin-HRP Complex (100x)
Biotinylated Detection Antibody Diluent
Avidin-Biotin-HRP Complex Diluent
Wash Buffer (20x)
Adhesive Plate Sealers
SPARC (secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.
Store at 4°C for 6 months or at -20°C for one year. Avoid freeze/thaw cycles.
SPARC ElisaKit, Basement-membrane protein 40 ElisaKit, BM-40 ElisaKit, Cysteine-rich protein ElisaKit, ON ElisaKit, Osteonectin ElisaKit
SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules.