Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The antibody pair provided in this kit recognizes human NB / NBL1.
LS-F437 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human NBL1 / DAN in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of NBL1 / DAN as low as 27.43 picograms per milliliter.
Cell Culture Supernatants, Plasma, Serum
Colorimetric - 450nm (TMB)
27.43 - 6667 pg/ml
Intra-Assay: CV<10% Inter-Assay: CV<12%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Coated 96-well Strip Plate
Wash Buffer Concentrate (20X)
Biotinylated Detection Antibody
HRP-Streptavidin Conjugate (300x)
TMB One-Step Substrate Reagent
Assay Diluent A
Assay Diluent B (5x)
Adhesive Plate Sealers
May be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standard should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze/thaw cycles.
NBL1 ElisaKit, DAN domain family member 1 ElisaKit, DAN ElisaKit, DAND1 ElisaKit, D1S1733E ElisaKit, NB ElisaKit, NO3 ElisaKit, Zinc finger protein DAN ElisaKit, Protein N03 ElisaKit
NB / NBL1 is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development.