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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human Leptin ELISA Kit (Sandwich ELISA) - LS-F2495
Catalog
Size
Price
LS-F2495-1
1 plate
$460

Popular Leptin Elisa Kits

Reactivity: Dog
Range: 0.313-20 ng/ml
Reactivity: Pig
Range: 31.2-2000 pg/ml
Reactivity: Mouse
Range: 4.096-1000 pg/ml
Reactivity: Pig
Range: 0.313-20 ng/ml
Reactivity: Human
Range: 0.156-10 ng/ml

Product Description

LS-F2495 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Leptin in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Leptin as low as 10 picograms per milliliter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
Leptin
Synonyms
LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB
Reactivity
Human
Specificity
This kit is for the detection of natural and recombinant human Leptin. No significant cross-reactivity or interference between Leptin and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
62.5 - 4000 pg/ml
Sensitivity
10 pg/ml
Precision
Intra-Assay: CV%<7.6% Inter-Assay: CV%<8.4%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody (100x)
  • ABC Complex (100x)
  • Sample Diluent
  • Detection Antibody Diluent
  • ABC Complex Diluent
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Restrictions
For research use only.
About Leptin
Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. P41159 NM_000230 NP_000221.1


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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 10/17/2018

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