LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Research Areas
  • Cancer Research:
  • Breast Cancer IHC
  • Colorectal Cancer IHC
  • Lung Cancer IHC
  • Prostate Cancer IHC
  • Cell Cycle:
  • Apoptosis
  • Protein Family and Group:
  • Caspases
  • Chemokines
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Human KRT20 / CK20 / Cytokeratin 20 ELISA Kit (Sandwich ELISA) - LS-F36651

Catalog Size Price
LS-F36651-1 1 plate $790

Most Popular KRT20 / CK20 / Cytokeratin 20 Elisa Kits

Mouse/Human/Rat KRT20 / CK20 / Cytokeratin 20 ELISA Kit (Cell-Based ELISA) - LS-F2105
Cell-Based ELISA
96-Well Microplate
Mouse, Human, Rat
Colorimetric - 450nm (TMB)
Adherent Cell Cultures
Human KRT20 / CK20 / Cytokeratin 20 ELISA Kit (Sandwich ELISA) - LS-F4499
Sandwich ELISA
96-Well Strip Plate
Human
0.156-10 ng/ml
Colorimetric - 450nm (TMB)
Serum, Tissue Homogenates, Plasma
Rat KRT20 / CK20 / Cytokeratin 20 ELISA Kit (Custom ELISA) - LS-F16575
Custom ELISA
96-Well Strip Plate
Rat
Colorimetric - 450nm (TMB)
Mouse KRT20 / CK20 / Cytokeratin 20 ELISA Kit (Custom ELISA) - LS-F16576
Custom ELISA
96-Well Strip Plate
Mouse
Colorimetric - 450nm (TMB)
Human KRT20 / CK20 / Cytokeratin 20 ELISA Kit (Custom ELISA) - LS-F16577
Custom ELISA
96-Well Strip Plate
Human
Colorimetric - 450nm (TMB)

100% Guaranteed
LS-F36651
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
KRT20 / CK20 / Cytokeratin 20
Human
LS-F36651 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human KRT20 / CK20 / Cytokeratin 20 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of KRT20 / CK20 / Cytokeratin 20 as low as 0.094 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.156 - 10 ng/ml
0.094
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CK- 20/KRT20 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CK- 20/KRT20 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CK-20/KRT20 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CK- 20/KRT20 can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About KRT20 / CK20 / Cytokeratin 20

P35900 X73502 CAA51914.1

KRT20 ElisaKit, Cytokeratin-20 ElisaKit, Cytokeratin 20 ElisaKit, CK20 ElisaKit, K20 ElisaKit, Keratin 20 ElisaKit, Keratin-20 ElisaKit, KRT21 ElisaKit, CK-20 ElisaKit, Protein IT ElisaKit

KRT20 / CK20 / Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 7/23/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number