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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human HSPA1A ELISA Kit (Sandwich ELISA) - LS-F4418
LS-F4418 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human HSPA1A in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of HSPA1A as low as 0.61 nanograms per millilter.
Catalog
Size
Price
LS-F4418-1
1 plate
$630
Available Discounts
10% off 5+ units

Popular HSPA1A Elisa Kits

Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Pig
Range: 1.56-100 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Bovine
Range: 1.56-100 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Bovine
Range: 0.78-50 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Product Description

LS-F4418 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human HSPA1A in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of HSPA1A as low as 0.61 nanograms per millilter.
About HSPA1A
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. NM_005345 NP_005336.3

HSPA1A ElisaKit, Heat shock 70kDa protein 1A ElisaKit, Heat shock 70 kDa protein 1/2 ElisaKit, Heat shock 70kD protein 1A ElisaKit, HSP70-1/HSP70-2 ElisaKit, HSPA1 ElisaKit, HSP70-1A ElisaKit, Heat shock 70 kd protein 1 ElisaKit, Heat shock-induced protein ElisaKit, Iroquois homeobox protein 4 ElisaKit, HSP70-1 ElisaKit, Hsp70.1 ElisaKit, HSP70.1/HSP70.2 ElisaKit, HSP70I ElisaKit, HSP72 ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
HSPA1A
Reactivity
Human
Specificity
Heat Shock 70kDa Protein 1A (HSPA1A) No significant cross-reactivity or interference between Heat Shock 70kDa Protein 1A (HSPA1A) and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Detection Range
1.56 - 100 ng/ml
Sensitivity
0.61 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Adhesive Plate Sealers
  • Standard (Lyophilized)
  • Sample Diluent
  • Detection Reagent A (Green)
  • Detection Reagent B (Red)
  • Assay Diluent A
  • Assay Diluent B
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (30x concentrate)
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/16/2018

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