Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
LS-F39249 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human GP78 / AMFR in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of GP78 / AMFR as low as 0.375 nanograms per millilter.
AMFR, AMF receptor, isoform 1, AMF receptor, isoform 2, gp78, RNF45, RING finger protein 45
Intended Sample Types
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
0.625 - 40 ng/ml
Intra-Assay: CV<8% Inter-Assay: CV<10%
Store at 4°C. Stable for 6 months.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Coated 96-well Strip Plate
Detection Antibody Diluent
Biotinylated Detection Antibody (100x)
HRP-Streptavidin Conjugate (100x)
HRP Streptavidin Conjugate Diluent
Wash Buffer (25x)
Adhesive Plate Sealers
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with AMFR. During the reaction, AMFR in the sample or standard competes with a fixed amount of AMFR on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to AMFR. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AMFR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR.