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Human CD40L ELISA Kit (Sandwich ELISA) - LS-F36234

Catalog Size Price
LS-F36234-1 1 plate Unavailable

Most Popular CD40L Elisa Kits

Human CD40L ELISA Kit (Sandwich ELISA) - LS-F53
Quantitative Sandwich ELISA
96-Well Microplate
Human
8.23-6000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Plasma
Mouse CD40L ELISA Kit (Custom ELISA) - LS-F372
Quantitative Custom ELISA
96-Well Microplate
Mouse
Colorimetric - 450nm (TMB)
Human CD40L ELISA Kit (Sandwich ELISA) - LS-F964
Sandwich ELISA
12 x 8-Well Microstrips
Human
32-2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Serum, Plasma
Human CD40L ELISA Kit (Sandwich ELISA) - LS-F2585
Quantitative Sandwich ELISA
96-Well Microplate
Human
62.5-4000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Plasma
Mouse CD40L ELISA Kit (Sandwich ELISA) - LS-F5175
Sandwich ELISA
96-Well Strip Plate
Mouse
15.63-1000 pg/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Serum, Tissue Homogenates, Plasma

100% Guaranteed
LS-F36234
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
CD40L
Human
LS-F36234 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CD40L in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CD40L as low as 37.5 picograms per milliliter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
62.5 - 4000 pg/ml
37.5
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CD40Lantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CD40L antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD40L amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD40L can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About CD40L

P29965 NM_000074 NP_000065.1

CD40LG ElisaKit, CD154 ElisaKit, CD154 antigen ElisaKit, CD40-L ElisaKit, CD40 ligand ElisaKit, CD40L ElisaKit, gp39 ElisaKit, HIGM1 ElisaKit, IGM ElisaKit, IMD3 ElisaKit, T-BAM ElisaKit, TNFSF5 ElisaKit, CD40 antigen ligand ElisaKit, CD54 ElisaKit, HCD40L ElisaKit, T-B cell-activating molecule ElisaKit, T-cell antigen Gp39 ElisaKit, TNF-related activation protein ElisaKit

TNFSF5 is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in inability of immunoglobulin class switch and is associated with hyper IgM syndrome.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/21/2018

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