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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Human CD27 ELISA Kit (Sandwich ELISA) - LS-F2632
Catalog
Size
Price
LS-F2632-1
1 plate
$530

Popular CD27 Elisa Kits

Reactivity: Mouse
Range: 62.5-4000 pg/ml
Reactivity: Human
Range: 62.5-4000 pg/ml
Reactivity: Mouse
Range: 62.5-4000 pg/ml

Product Description

LS-F2632 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CD27 in samples of Cell Culture Supernatants and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of CD27 as low as 1 picograms per milliliter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CD27
Synonyms
CD27, CD27 antigen, CD27 molecule, S152, T14, T-cell activation antigen CD27, CD27L receptor, T cell activation antigen S152, TNFRSF7, Tp55
Reactivity
Human
Specificity
This kit is for the detection of natural and recombinant human TNFRSF7. No significant cross-reactivity or interference between TNFRSF7 and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
62.5 - 4000 pg/ml
Sensitivity
1 pg/ml
Precision
Intra-Assay: CV%<5.4% Inter-Assay: CV%<6.7%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody (100x)
  • ABC Complex (100x)
  • Sample Diluent
  • Detection Antibody Diluent
  • ABC Complex Diluent
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Restrictions
For research use only.
About CD27
CD27 is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. P26842 NM_001242 NP_001233.1


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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 10/17/2018

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