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Human ANGPTL3 ELISA Kit (Sandwich ELISA) - LS-F10527
Catalog
Size
Price
LS-F10527-1
1 plate
$530

Popular ANGPTL3 Elisa Kits

Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Monkey
Range: 39.063-2500 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Mouse
Range: 40.96-4000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 12 x 8-Well Microstrips
Reactivity: Human
Range: 32-2000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 3.13-200 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 0.625-40 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

Product Description

LS-F10527 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human ANGPTL3 in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of ANGPTL3 as low as 10 picograms per milliliter.
About ANGPTL3
ANGPTL3 encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metablism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2. Q9Y5C1 NM_014495 NP_055310.1

ANGPTL3 ElisaKit, ANGPT5 ElisaKit, ANG-5 ElisaKit, Angiopoietin-5 ElisaKit, Angiopoietin-like 3 ElisaKit, Angiopoietin-related protein 3 ElisaKit, FHBL2 ElisaKit, Angiopoietin 5 ElisaKit, Angiopoietin-like protein 3 ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
ANGPTL3
Reactivity
Human
Specificity
This kit is for the detection of natural and recombinant human ANGPTL3. No significant cross-reactivity or interference between ANGPTL3 and analogs was observed.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
312 - 20000 pg/ml
Sensitivity
10 pg/ml
Precision
Intra-Assay: CV%<4.7% Inter-Assay: CV%<7.4%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody (100x)
  • ABC Complex (100x)
  • Sample Diluent
  • Detection Antibody Diluent
  • ABC Complex Diluent
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Background
ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
Restrictions
For research use only.

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Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 9/23/2018

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