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Human Angiogenin / ANG ELISA Kit (Sandwich ELISA) - LS-F24352

Catalog Size Price
LS-F24352-1 1 plate Unavailable

Most Popular Angiogenin / ANG Elisa Kits

Human Angiogenin / ANG ELISA Kit (Sandwich ELISA) - LS-F30
Quantitative Sandwich ELISA
96-Well Microplate
Human
1.638-160 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Plasma
 Image
Human Angiogenin / ANG ELISA Kit (Sandwich ELISA) - LS-F674
Sandwich ELISA
12 x 8-Well Microstrips
Human
16-500 pg/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Serum, Plasma
 Image
Mouse Angiogenin / ANG ELISA Kit (Sandwich ELISA) - LS-F10821
Quantitative Sandwich ELISA
96-Well Strip Plate
Mouse
15.6-1000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma
 Image
Pig Angiogenin / ANG ELISA Kit (Sandwich ELISA) - LS-F22393
Quantitative Sandwich ELISA
96-Well Microplate
Pig
0.313-20 ng/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
 Image
Rat Angiogenin / ANG ELISA Kit (Sandwich ELISA) - LS-F23895
Quantitative Sandwich ELISA
96-Well Microplate
Rat
62.5-4000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
 Image

100% Guaranteed
LS-F24352
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Angiogenin / ANG
Human
For the detection and quantification of endogenous levels of natural and/or recombinant Human ANG proteins.
LS-F24352 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Angiogenin / ANG in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Angiogenin / ANG as low as 12 picograms per milliliter.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Cell Culture Supernatants, Plasma, Serum
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
78 - 5000 pg/ml
12 pg/ml
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody (100x)
  • Avidin-Biotin-HRP Complex (100x)
  • Sample Diluent
  • Biotinylated Detection Antibody Diluent
  • Avidin-Biotin-HRP Complex Diluent
  • Wash Buffer (20x)
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
    Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
Store at 4°C for 6 months or at -20°C for one year. Avoid freeze/thaw cycles.
For research use only.

About Angiogenin / ANG

P03950 NM_001145 NP_001136.1

ANG Elisa Kit, Angiogenin Elisa Kit, HEL168 Elisa Kit, ALS9 Elisa Kit, Ribonuclease 5 Elisa Kit, RNASE5 Elisa Kit, Epididymis luminal protein 168 Elisa Kit, RNase 5 Elisa Kit

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues.

 

Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 6/27/2017

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