LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
Human AMY2A / Pancreatic Amylase ELISA Kit (Sandwich ELISA) - LS-F36188
Catalog
Size
Price
LS-F36188-1
1 plate
$790

Popular AMY2A / Pancreatic Amylase Elisa Kits

Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 3.13-200 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.156-10 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Sandwich CLIA ELISA
Format: 12 x 8-Well Microstrips
Reactivity: Human
Range: 0.625-40 ng/ml
Detection: Chemiluminescent
Sample Types: Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rat
Range: 1.563-100 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

Product Description

LS-F36188 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human AMY2A / Pancreatic Amylase in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of AMY2A / Pancreatic Amylase as low as 1.875 nanograms per millilter.
About AMY2A / Pancreatic Amylase
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. P04746 NM_000699 NP_000690.1

AMY2A ElisaKit, Alpha-amylase ElisaKit, Amylase, alpha 2A (pancreatic) ElisaKit, Found in the pancreas ElisaKit, Glycogenase ElisaKit, Pancreatic alpha-amylase ElisaKit, Pancreatic Amylase ElisaKit, Pancreatic amylase 2A ElisaKit, AMY2 ElisaKit, Amylase, alpha 2A pancreatic ElisaKit, Amylase, pancreatic, alpha-2A ElisaKit, Pancreatic amylase alpha 2A ElisaKit, Pa ElisaKit


Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
AMY2A / Pancreatic Amylase
Reactivity
Human
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
3.125 - 200 ng/ml
Sensitivity
1.875 ng/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AMY2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AMY2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AMY2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AMY2 can be calculated.
Restrictions
For research use only.

Publications (0)


Customer Reviews (0)


Images

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 9/20/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy