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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Fish Estradiol ELISA Kit (Competitive EIA) - LS-F10121
LS-F10121 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Fish Estradiol in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Estradiol as low as 40 picograms per milliliter.
Catalog
Size
Price
LS-F10121-1
1 plate
$620
Available Discounts
10% off 5+ units

Popular Estradiol Elisa Kits

Type: Competitive EIA
Format: 96-Well Strip Plate
Range: 12.35-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Quantitative Competitive EIA
Format: 96-Well Microplate
Reactivity: Human
Range: 40-1500 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, Urine, Plasma
Type: Quantitative Competitive EIA
Format: 96-Well Microplate
Reactivity: Mouse
Range: 40-1500 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma
Type: Quantitative Competitive EIA
Format: 96-Well Microplate
Reactivity: Rat
Range: 40-1500 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma
Type: Quantitative Competitive EIA
Format: 96-Well Microplate
Reactivity: Goat
Range: 40-1400 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

Product Description

LS-F10121 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Fish Estradiol in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Estradiol as low as 40 picograms per milliliter.

Specifications

Type
Competitive EIA (enzyme immunoassay) kit
Target
Estradiol
Reactivity
Fish
Specificity
This assay has high sensitivity and excellent specificity for detection of fish E2. No significant cross-reactivity or interference between fish E2 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between fish E2 and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
40 - 1500 pg/ml
Sensitivity
40 pg/ml
Precision
Intra-assay: CV%<15% Inter-assay: CV%<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Assay plate
  • 6 x Standard
  • Antibody
  • HRP-conjugate
  • Wash Buffer (20 x concentrate)
  • Substrate A
  • Substrate B
  • Stop Solution
  • Adhesive Strip (For 96 wells)
Background
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: United States
Date Requested: 8/16/2018

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