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Oxidative DNA Damage / 8-OH-dG ELISA Kit (Competitive EIA) - LS-F20632 Best Seller
Catalog
Size
Price
LS-F20632-1
1 plate
$580

Product Description

LS-F20632 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Oxidative DNA Damage / 8-OH-dG in samples of Cell Lysates, Plasma and Urine. It is based upon a Competitive EIA assay principle and can be used to detect levels of Oxidative DNA Damage / 8-OH-dG as low as 0.59 nanograms per millilter.

Specifications

Type
Competitive EIA (enzyme immunoassay) kit
Target
Oxidative DNA Damage / 8-OH-dG
Manual
Intended Sample Types
Cell Lysates, Plasma, Urine
Format
96-Well Strip Plate
Detection
Colorimetric - 420nm (Ellman's)
Measurement
Quantitative
Detection Range
0.94 - 60 ng/ml
Sensitivity
0.59 ng/ml
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • User Manual
  • 96-well Microplate
  • Detection Reagents
  • Wash Buffer
  • Substrate
  • Stop Solution
  • Adhesive Plate Sealers
Background
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OHdG have been reported to be between 4-21 pg/ml.
Restrictions
For research use only.

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Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: United States
Date Requested: 12/10/2018
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