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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-K554-1000 1000 asy $725 
Cell Viability Assay Kit - (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.

WST-NR-CV Combined Cytotoxicity Assay Kit (Colorimetric) - LS-K554

WST-NR-CV Combined Cytotoxicity Assay Kit (Colorimetric) - LS-K554

Available for shipment within the USA only
Description:
Cytotoxicity assays are essential tools in drug discovery. In vitro single parameter cytotoxicity assays, such as using tetrazolium salt (WST), neutral red (NR) or crystal violet (CV) in cultured cells, are widely used to assess the cytotoxicity of different compounds as these methods are fast, economical and animal-free. However, a major problem of using single parameter assays is determined IC50 data using these individual assays sometimes can be quite different due to specific compound inhibition. Consequently, if the cytotoxicity assay is not selected appropriately, false negative compounds that are actually cytotoxic may be easily missed. To partly overcome this problem, multiple parameter cytotoxicity assays are faster and more comprehensive alternatives to determine IC50 and cytotoxicity in different pathways. The WST-NR-CV Combined Cytotoxicity Assay Kit can allow researchers to test the cytotoxicity of their compounds in different pathways such as in cell proliferation (WST), lysosome activity (NR) and total DNA synthesis (CV). The kit is simple and fast, yet it provides a quantitative measurement of the number of viable cells, it offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening.
Price
Catalog Number
$725 
LS-K554-1000
1000 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
Cytotoxicity assays are essential tools in drug discovery. In vitro single parameter cytotoxicity assays, such as using tetrazolium salt (WST), neutral red (NR) or crystal violet (CV) in cultured cells, are widely used to assess the cytotoxicity of different compounds as these methods are fast, economical and animal-free. However, a major problem of using single parameter assays is determined IC50 data using these individual assays sometimes can be quite different due to specific compound inhibition. Consequently, if the cytotoxicity assay is not selected appropriately, false negative compounds that are actually cytotoxic may be easily missed. To partly overcome this problem, multiple parameter cytotoxicity assays are faster and more comprehensive alternatives to determine IC50 and cytotoxicity in different pathways. The WST-NR-CV Combined Cytotoxicity Assay Kit can allow researchers to test the cytotoxicity of their compounds in different pathways such as in cell proliferation (WST), lysosome activity (NR) and total DNA synthesis (CV). The kit is simple and fast, yet it provides a quantitative measurement of the number of viable cells, it offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening.

Specifications

Name
WST-NR-CV Combined Cytotoxicity Assay Kit (Colorimetric)
Type
Cytotoxicity Assay
Usage
The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
Target
Cell Viability
SampleType
Adherent Cells, Non-adherent Cells
Detection
Colorimetric (WST/NR/CV 450/540/570 nm Absorbance)
Species Reactivity
Eukaryote
Components
  • WST Reagent (lyophilized)
  • WST Developing Solution
  • 100X NR Staining Solution
  • 2X NR Solubilization Solution
  • CV Staining Solution
  • 10X CV Solubilization Solution
  • 10X Washing Solution
  • 20 mM Doxorubicin
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Components stored at varying temperatures. See recommended storage details in product insert, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Convenient
    • Entire assay can be performed directly in a 96-well plate
    • Method is simple and does not require extensive washing/harvesting/or solubilization steps
    • Simple procedure
    • Takes less than 4 hours
    • Uses a water-soluble Tetrazolium salt WST reagent, Neutral Red, and Crystal Violet

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    Customer Reviews (0)

    Images

    Assay Result

    Cell Viability Assay Kit - (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.
    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.

    Assay Result

    Cell Viability Assay Kit - (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.
    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.

    Assay Result

    Cell Viability Assay Kit - (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.
    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.

    Assay Result

    Cell Viability Assay Kit - (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.
    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.

    Requested From: United States
    Date Requested: 7/4/2020