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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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WST-NR-CV Combined Cytotoxicity Assay Kit (Colorimetric) - LS-K554

Available for shipment within the USA only
Catalog
Size
Price
LS-K554-1000
1000 asy
$860

Available for shipment within the USA only

Product Description

Cytotoxicity assays are essential tools in drug discovery. In vitro single parameter cytotoxicity assays, such as using tetrazolium salt (WST), neutral red (NR) or crystal violet (CV) in cultured cells, are widely used to assess the cytotoxicity of different compounds as these methods are fast, economical and animal-free. However, a major problem of using single parameter assays is determined IC50 data using these individual assays sometimes can be quite different due to specific compound inhibition. Consequently, if the cytotoxicity assay is not selected appropriately, false negative compounds that are actually cytotoxic may be easily missed. To partly overcome this problem, multiple parameter cytotoxicity assays are faster and more comprehensive alternatives to determine IC50 and cytotoxicity in different pathways. The WST-NR-CV Combined Cytotoxicity Assay Kit can allow researchers to test the cytotoxicity of their compounds in different pathways such as in cell proliferation (WST), lysosome activity (NR) and total DNA synthesis (CV). The kit is simple and fast, yet it provides a quantitative measurement of the number of viable cells, it offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening.

Specifications

Name
WST-NR-CV Combined Cytotoxicity Assay Kit (Colorimetric)
Product Type
Cytotoxicity Assay
Usage Summary
The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
Target
Cell Viability
Sample Type
Adherent Cells, Non-adherent Cells
Detection
Colorimetric (WST/NR/CV 450/540/570 nm Absorbance)
Species Reactivity
Eukaryote
Kit Components
  • WST Reagent (lyophilized)
  • WST Developing Solution
  • 100X NR Staining Solution
  • 2X NR Solubilization Solution
  • CV Staining Solution
  • 10X CV Solubilization Solution
  • 10X Washing Solution
  • 20 mM Doxorubicin
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C for up to 1 year, 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Customer Reviews (0)


    Images

    Assay Result

    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.
    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.

    Assay Result

    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.
    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.

    Assay Result

    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.
    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.

    Assay Result

    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.
    (a) Dose-response curves of Doxorubicin detected by the assay kit in the MCF-7 cells. (b) Dose-response curves of Triton X-100 detected by the assay kit in the MCF-7 cells.

    Requested From: United States
    Date Requested: 9/15/2019