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Catalog Number Size Price
LS-K225-100 100 Tests $415 
Urea Assay Kit

Urea Assay Kit III (Colorimetric) - LS-K225

Urea Assay Kit III (Colorimetric) - LS-K225

Available for shipment within the USA only
Description:
UREA, the major end product of protein catabolism in animals, is primarily produced in the liver and secreted by the kidneys. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is very useful for medical clinicians to assess kidney function of patients. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases (e.g. congestive heart failure, liver diseases, and diabetes). Decreased levels often indicate acute hepatic insufficiency, but may also result from over vigorous parenteral fluid therapy. Simple, direct and automation-ready procedures for measuring urea or blood urea nitrogen (BUN) are popular in research and drug discovery. This urea assay is designed to directly measure urea in biological samples. In this assay, urease converts urea to ammonia and carbon dioxide. NADH is then converted to NAD+ in the presence of ammonia, Alpha-ketoglutarate, and glutamate dehydrogenase. The decrease in optical density at 340 nm is directly proportional to the urea concentration in the sample.
Price
Catalog Number
$415
LS-K225-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
UREA, the major end product of protein catabolism in animals, is primarily produced in the liver and secreted by the kidneys. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is very useful for medical clinicians to assess kidney function of patients. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases (e.g. congestive heart failure, liver diseases, and diabetes). Decreased levels often indicate acute hepatic insufficiency, but may also result from over vigorous parenteral fluid therapy. Simple, direct and automation-ready procedures for measuring urea or blood urea nitrogen (BUN) are popular in research and drug discovery. This urea assay is designed to directly measure urea in biological samples. In this assay, urease converts urea to ammonia and carbon dioxide. NADH is then converted to NAD+ in the presence of ammonia, Alpha-ketoglutarate, and glutamate dehydrogenase. The decrease in optical density at 340 nm is directly proportional to the urea concentration in the sample.

Specifications

Name
Urea Assay Kit III (Colorimetric)
Type
Detection/Quantition
Usage
For quantitative enzymatic determination of urea.
Target
Urea
SampleType
Biological Samples, Food and Beverages
Detection
Colorimetric (340 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • Enzyme
  • Ketoglutarate
  • Urease
  • NADH Reagent: Dried Standard
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible)
Conditions
Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

Urea Assay Kit

Colorimetry

Urea Assay Kit

Colorimetry

Urea Assay Kit

Colorimetry

Urea Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/19/2024