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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Urea Assay Kit III (Colorimetric)

Available for shipment within the USA only
Catalog
Size
Price
LS-K225-100
100 tst
$260

Available for shipment within the USA only

Product Description

UREA, the major end product of protein catabolism in animals, is primarily produced in the liver and secreted by the kidneys. It is the primary vehicle for removal of toxic ammonia from the body. Urea determination is very useful for medical clinicians to assess kidney function of patients. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases (e.g. congestive heart failure, liver diseases, and diabetes). Decreased levels often indicate acute hepatic insufficiency, but may also result from over vigorous parenteral fluid therapy. Simple, direct and automation-ready procedures for measuring urea or blood urea nitrogen (BUN) are popular in research and drug discovery. This urea assay is designed to directly measure urea in biological samples. In this assay, urease converts urea to ammonia and carbon dioxide. NADH is then converted to NAD+ in the presence of ammonia, Alpha-ketoglutarate, and glutamate dehydrogenase. The decrease in optical density at 340 nm is directly proportional to the urea concentration in the sample.

Specifications

Name
Urea Assay Kit III (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
For quantitative enzymatic determination of urea.
Target
Urea
Sample Type
Biological Samples, Food and Beverages
Detection
Colorimetric (340 nm Absorbance)
Kit Components
  • Assay Buffer
  • Enzyme
  • Ketoglutarate
  • Urease
  • NADH Reagent: Dried Standard
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 6 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Requested From: United States
    Date Requested: 1/21/2019
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