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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Triglyceride Quantification Kit (Colorimetric/Fluorometric) - LS-K126
Available for shipment within the USA only
Catalog
Size
Price
LS-K126-100
100 asy
$615

Available for shipment within the USA only

Product Description

Triglycerides (TG) are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. TG are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis. The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In the assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a product which reacts with the probe to generate color (spectrophotometry at ? = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 2 pmol-10nmol (or 2-10000µM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.

Specifications

Name
Triglyceride Quantification Kit (Colorimetric/Fluorometric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection and measurement of monoglycerides and diglycerides in addition to triglycerides in various biological samples.
Target
Triglyceride
Sample Type
Biological Fluids, Plasma, Serum, Tissue Culture Supernatants, Urine
Detection
Colorimetric (570 nm Absorbance), Fluorometric (Ex/Em 535/587 nm)
Species Reactivity
Mammal
Kit Components
  • Triglyceride Assay Buffer
  • TriglycerideProbe (in DMSO anhydrous)
  • Lipase
  • Triglyceride Enzyme Mix (lyophilized)
  • Triglyceride Standard (1 mM)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric), Spectrophotometry (visible)
    Equipment
    Microplate fluorometer, Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    (a) Triglyceride Quantification Kit standard curve. (b) Determination of Triglyceride in pooled normal human serum. Serum sample (3 µl) was spiked with a known amount of Triglyceride as internal Standard (4 nmol ).
    (a) Triglyceride Quantification Kit standard curve. (b) Determination of Triglyceride in pooled normal human serum. Serum sample (3 µl) was spiked with a known amount of Triglyceride as internal Standard (4 nmol ).

    Assay Result

    (a) Triglyceride Quantification Kit standard curve. (b) Determination of Triglyceride in pooled normal human serum. Serum sample (3 µl) was spiked with a known amount of Triglyceride as internal Standard (4 nmol ).
    (a) Triglyceride Quantification Kit standard curve. (b) Determination of Triglyceride in pooled normal human serum. Serum sample (3 µl) was spiked with a known amount of Triglyceride as internal Standard (4 nmol ).

    Assay Result

    (a) Triglyceride Quantification Kit standard curve. (b) Determination of Triglyceride in pooled normal human serum. Serum sample (3 µl) was spiked with a known amount of Triglyceride as internal Standard (4 nmol ).
    (a) Triglyceride Quantification Kit standard curve. (b) Determination of Triglyceride in pooled normal human serum. Serum sample (3 µl) was spiked with a known amount of Triglyceride as internal Standard (4 nmol ).

    Assay Result

    (a) Triglyceride Quantification Kit standard curve. (b) Determination of Triglyceride in pooled normal human serum. Serum sample (3 µl) was spiked with a known amount of Triglyceride as internal Standard (4 nmol ).
    (a) Triglyceride Quantification Kit standard curve. (b) Determination of Triglyceride in pooled normal human serum. Serum sample (3 µl) was spiked with a known amount of Triglyceride as internal Standard (4 nmol ).

    Requested From: United States
    Date Requested: 12/13/2018
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