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Catalog Number Size Price
LS-K224-100 100 Tests $470 
Superoxide Dismutase Assay Kit

Superoxide Dismutase (SOD) Assay Kit (Colorimetric) - LS-K224

Superoxide Dismutase (SOD) Assay Kit (Colorimetric) - LS-K224

Available for shipment within the USA only
Description:
Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide into O2 and H2O2. They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer. This SOD assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a WST-1 dye to form a colored product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.
Price
Catalog Number
$470
LS-K224-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide into O2 and H2O2. They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer. This SOD assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a WST-1 dye to form a colored product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.

Specifications

Name
Superoxide Dismutase (SOD) Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
For quantitative determination of superoxide dismutase (SOD) enzyme activity.
Target
Superoxide Dismutase
SampleType
Blood, Cells, Tissue
Detection
Colorimetric (440 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • Diluent
  • SOD Enzyme
  • XO Enzyme
  • Xanthine
  • WST-1
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible)
Conditions
Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

Superoxide Dismutase Assay Kit

Colorimetry

Superoxide Dismutase Assay Kit

Colorimetry

Superoxide Dismutase Assay Kit

Colorimetry

Superoxide Dismutase Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/19/2024