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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-K752-1000 1000 asy $330 
Cell Viability Assay Kit - Dose-response curves of HepG2 (a), MCF-7 (b) and HEK293 (c) cells after exposing to doxorubicin for 72 hours determined by the SRB assay.

Sulforhodamine B (SRB) Cell Cytotoxicity Assay Kit (Color) - LS-K752

Sulforhodamine B (SRB) Cell Cytotoxicity Assay Kit (Color) - LS-K752

Available for shipment within the USA only
Description:
Sulforhodamine B (SRB) cell cytotoxicity assay is one of the most widely methods used to detect cell viability or drug cytotoxicity. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass. SRB is a bright-pink aminoxanthene dye that can form an electrostatic complex with basic amino acid residues of proteins in slightly acidic conditions but it can dissociate under basic conditions. It has been widely used for drug toxicity screening against different types of cancerous and non-cancerous cell lines. In addition, this assay is independent of cell metabolic activity and therefore should show less interference by the testing compounds. Since the binding of SRB is stoichiometric, the incorporated dye released from stained cells after washing is directly proportional to the cell biomass and can be measured at 565 nm. The SRB cell cytotoxicity assay kit is simple, accurate, reproducible and sensitive. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5,000-50,000 cells per well
Price
Catalog Number
$330 
LS-K752-1000
1000 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Sulforhodamine B (SRB) cell cytotoxicity assay is one of the most widely methods used to detect cell viability or drug cytotoxicity. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass. SRB is a bright-pink aminoxanthene dye that can form an electrostatic complex with basic amino acid residues of proteins in slightly acidic conditions but it can dissociate under basic conditions. It has been widely used for drug toxicity screening against different types of cancerous and non-cancerous cell lines. In addition, this assay is independent of cell metabolic activity and therefore should show less interference by the testing compounds. Since the binding of SRB is stoichiometric, the incorporated dye released from stained cells after washing is directly proportional to the cell biomass and can be measured at 565 nm. The SRB cell cytotoxicity assay kit is simple, accurate, reproducible and sensitive. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5,000-50,000 cells per well

Specifications

Name
Sulforhodamine B (SRB) Cell Cytotoxicity Assay Kit (Color)
Type
Cytotoxicity Assay
Usage
This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of SRB to bind cellular protein components and measure the total biomass.
Target
Cell Viability
SampleType
Adherent Cell Culture
Detection
Colorimetric (565 nm Absorbance)
Species Reactivity
Mammal
Components
  • Fixation Solution
  • 20X Washing Solution
  • 10X Solubilization Solution
  • SRB Dye Solution
  • 20 mM Doxorubicin
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Components stored at varying temperatures. See recommended storage details in product insert, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Fast, sensitive, and convenient
    • Simple procedure
    • Takes less than 2 hours

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    Customer Reviews (0)

    Images

    Assay Result

    Cell Viability Assay Kit - Dose-response curves of HepG2 (a), MCF-7 (b) and HEK293 (c) cells after exposing to doxorubicin for 72 hours determined by the SRB assay.
    Dose-response curves of HepG2 (a), MCF-7 (b) and HEK293 (c) cells after exposing to doxorubicin for 72 hours determined by the SRB assay.

    Assay Result

    Cell Viability Assay Kit - Dose-response curves of HepG2 (a), MCF-7 (b) and HEK293 (c) cells after exposing to doxorubicin for 72 hours determined by the SRB assay.
    Dose-response curves of HepG2 (a), MCF-7 (b) and HEK293 (c) cells after exposing to doxorubicin for 72 hours determined by the SRB assay.

    Assay Result

    Cell Viability Assay Kit - Dose-response curves of HepG2 (a), MCF-7 (b) and HEK293 (c) cells after exposing to doxorubicin for 72 hours determined by the SRB assay.
    Dose-response curves of HepG2 (a), MCF-7 (b) and HEK293 (c) cells after exposing to doxorubicin for 72 hours determined by the SRB assay.

    Assay Result

    Cell Viability Assay Kit - Dose-response curves of HepG2 (a), MCF-7 (b) and HEK293 (c) cells after exposing to doxorubicin for 72 hours determined by the SRB assay.
    Dose-response curves of HepG2 (a), MCF-7 (b) and HEK293 (c) cells after exposing to doxorubicin for 72 hours determined by the SRB assay.

    Requested From: United States
    Date Requested: 12/10/2019