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Catalog Number Size Price
LS-K190-100 100 Tests $470 
Phospholipase D Assay Kit

Phospholipase D (PLD) Assay Kit (Colorimetric/Fluorometric) - LS-K190

Phospholipase D (PLD) Assay Kit (Colorimetric/Fluorometric) - LS-K190

Available for shipment within the USA only
Description:
Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. This Method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.
Price
Catalog Number
$470
LS-K190-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. This Method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.

Specifications

Name
Phospholipase D (PLD) Assay Kit (Colorimetric/Fluorometric)
Type
Detection/Quantition
Usage
For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
Target
Phospholipase D
SampleType
Biological Samples
Detection
Colorimetric (570 nm Absorbance), Fluorometric (Ex/Em 530/585 nm)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • Dye Reagent
  • Enzyme Mix: Dried Substrate
  • Calibrator
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (fluorometric), Spectrophotometry (visible)
Equipment
Microplate fluorometer, Microplate spectrophotometer (visible)
Conditions
Shipped -20°C Dry Ice, Store at -20°C, 3 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

Phospholipase D Assay Kit

Colorimetry

Phospholipase D Assay Kit

Colorimetry

Phospholipase D Assay Kit

Colorimetry

Phospholipase D Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024