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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LDH-Cytotoxicity Assay Kit (Colorimetric) - LS-K1

Available for shipment within the USA only
Catalog
Size
Price
LS-K1-400
400 asy
$390

Available for shipment within the USA only

Product Description

Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. The LDH-Cytotoxicity Assay Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm. The LDH-cytotoxicity assay is sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies.

Specifications

Name
LDH-Cytotoxicity Assay Kit (Colorimetric)
Product Type
Cytotoxicity Assay
Usage Summary
This assay provides a convenient and non-radioactive alternative to the [3H]-thymidine and the [51Cr] release assays. The assay is based on measurement of activity of lactate dehydrogenase (LDH) which is a stable enzyme normally found in the cytosol of all cells but rapidly releases into the supernatant upon damage of plasma membrane.
Target
Cell Viability
Sample Type
Cell Cultures
Detection
Colorimetric (500 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • Catalyst (Lyophilized)
  • Dye Solution
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    Jurkat cells were cultured in 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium. Light bar: Low control; Dark bar: High control.
    Jurkat cells were cultured in 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium. Light bar: Low control; Dark bar: High control.

    Assay Result

    Jurkat cells were cultured in 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium. Light bar: Low control; Dark bar: High control.
    Jurkat cells were cultured in 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium. Light bar: Low control; Dark bar: High control.

    Assay Result

    Jurkat cells were cultured in 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium. Light bar: Low control; Dark bar: High control.
    Jurkat cells were cultured in 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium. Light bar: Low control; Dark bar: High control.

    Assay Result

    Jurkat cells were cultured in 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium. Light bar: Low control; Dark bar: High control.
    Jurkat cells were cultured in 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium. Light bar: Low control; Dark bar: High control.

    Requested From: United States
    Date Requested: 6/19/2019
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