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Catalog Number Size Price
LS-K35-100 100 asy $515 
Invertase Assay Kit - Detection of bacterial Invertase activity in human saliv(a) Samples collected: 1- Fasting; 2- Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 µl and 3 = 10 µl) were incubated for 20 min. with Invertase substrate.

Invertase Activity Kit (Colorimetric) - LS-K35

Invertase Activity Kit (Colorimetric) - LS-K35

Available for shipment within the USA only
Description:
Invertase (EC 3.2.1.26), also known as sucrase or beta-fructofuranosidase, catalyzes the hydrolysis of sucrose (table sugar) by cleaving its glycosidic bond and forming one molecule each of glucose and fructose. Invertase is widely expressed among plants and microorganisms including yeast and bacteria. It is extensively utilized in industrial processes, including food, ethanol production, cosmetics, drugs, paper and bioelectronics electrodes. This Invertase Activity Assay kit provides a simple, sensitive and high-throughput adaptable assay that detects relevant concentrations of Invertase in biological samples or purified/crude enzyme preparations. The assay is based on the enzymatic detection of glucose, one of the hydrolysis products. Detection limit: 20 µU.
Price
Catalog Number
$515 
LS-K35-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Invertase (EC 3.2.1.26), also known as sucrase or beta-fructofuranosidase, catalyzes the hydrolysis of sucrose (table sugar) by cleaving its glycosidic bond and forming one molecule each of glucose and fructose. Invertase is widely expressed among plants and microorganisms including yeast and bacteria. It is extensively utilized in industrial processes, including food, ethanol production, cosmetics, drugs, paper and bioelectronics electrodes. This Invertase Activity Assay kit provides a simple, sensitive and high-throughput adaptable assay that detects relevant concentrations of Invertase in biological samples or purified/crude enzyme preparations. The assay is based on the enzymatic detection of glucose, one of the hydrolysis products. Detection limit: 20 µU.

Specifications

Name
Invertase Activity Kit (Colorimetric)
Type
Enzymatic Activity Assay
Usage
This assay provides for the detection of Invertase activity in various biological fluids, bacteria and crude enzyme preparations. Applicable for the analysis of metabolic pathways such as glycolysis and Diabetes Obesity-related Signaling.
Target
Invertase
SampleType
Biological Fluids
Detection
Colorimetric (570 nm Absorbance)
Species Reactivity
Mammal, Bacteria
Components
  • Invertase Hydrolysis Buffer
  • Invertase Assay Buffer
  • Invertase Stop Solution
  • OxiRedTM Probe (in DMSO)
  • Invertase Enzyme Mix (lyophilized)
  • Invertase Substrate
  • Glucose Standard (100 mM)
  • Positive Control (lyophilized)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Simple and rapid protocol
    • Highly sensitive: Detects as low as 20 uU Invertase in various sample types

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    Images

    Assay Result

    Invertase Assay Kit - Detection of bacterial Invertase activity in human saliv(a) Samples collected: 1- Fasting; 2- Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 µl and 3 = 10 µl) were incubated for 20 min. with Invertase substrate.
    Detection of bacterial Invertase activity in human saliv(a) Samples collected: 1- Fasting; 2- Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 µl and 3 = 10 µl) were incubated for 20 min. with Invertase substrate.

    Assay Result

    Invertase Assay Kit - Detection of bacterial Invertase activity in human saliv(a) Samples collected: 1- Fasting; 2- Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 µl and 3 = 10 µl) were incubated for 20 min. with Invertase substrate.
    Detection of bacterial Invertase activity in human saliv(a) Samples collected: 1- Fasting; 2- Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 µl and 3 = 10 µl) were incubated for 20 min. with Invertase substrate.

    Assay Result

    Invertase Assay Kit - Detection of bacterial Invertase activity in human saliv(a) Samples collected: 1- Fasting; 2- Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 µl and 3 = 10 µl) were incubated for 20 min. with Invertase substrate.
    Detection of bacterial Invertase activity in human saliv(a) Samples collected: 1- Fasting; 2- Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 µl and 3 = 10 µl) were incubated for 20 min. with Invertase substrate.

    Assay Result

    Invertase Assay Kit - Detection of bacterial Invertase activity in human saliv(a) Samples collected: 1- Fasting; 2- Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 µl and 3 = 10 µl) were incubated for 20 min. with Invertase substrate.
    Detection of bacterial Invertase activity in human saliv(a) Samples collected: 1- Fasting; 2- Low calorie meal and 3: High Sucrose meal. Undiluted samples (1 and 2 = 25 µl and 3 = 10 µl) were incubated for 20 min. with Invertase substrate.

    Requested From: United States
    Date Requested: 2/17/2020