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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GST Activity Kit (Colorimetric) - LS-K4

Available for shipment within the USA only
Catalog
Size
Price
LS-K4-100
100 asy
$500

Available for shipment within the USA only

Product Description

Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. The GST Colorimetric Activity Assay Kit is based upon the GST-catalyzed reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene, which has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). Under certain conditions, the interaction between glutathione and CDNB is totally dependent on the presence of active GST. The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm. One unit of GST activity is defined as the amount of enzyme producing 1 µmol of GS-DNB conjugate/min under the conditions of the assay. The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.

Specifications

Name
GST Activity Kit (Colorimetric)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay measures Glutathione S-transferase (GST) activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
Target
Glutathione S-Transferase
Sample Type
Crude Cell Lysates, Purified Protein Fractions
Detection
Colorimetric (380 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • GST Assay Buffer
  • GST Substrate (CDNB)
  • Glutathione (GSH -lyophilized)
  • GST Positive Control (0.625 -¦g/¦l)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (UV)
    Equipment
    Microplate spectrophotometer (UV)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About Glutathione S-Transferase
    O18598

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    Images

    Assay Result

    Measurement of GST sample activity.
    Measurement of GST sample activity.

    Assay Result

    Measurement of GST sample activity.
    Measurement of GST sample activity.

    Assay Result

    Measurement of GST sample activity.
    Measurement of GST sample activity.

    Assay Result

    Measurement of GST sample activity.
    Measurement of GST sample activity.

    Requested From: United States
    Date Requested: 4/19/2019
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