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Catalog Number Size Price
LS-K250-100 100 Tests $415 
Assay Kit

Glutathione S-transferase (GST) Assay Kit (Colorimetric) - LS-K250

Glutathione S-transferase (GST) Assay Kit (Colorimetric) - LS-K250

Available for shipment within the USA only
Description:
GLUTATHIONE TRANSFERASE (GST; EC 2.5.1.18) is a multifunctionalenzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites. This Glutathione S-transferase assay kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.
Price
Catalog Number
$415
LS-K250-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
GLUTATHIONE TRANSFERASE (GST; EC 2.5.1.18) is a multifunctionalenzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites. This Glutathione S-transferase assay kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.

Specifications

Name
Glutathione S-transferase (GST) Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
Glutathione S-transferase activity determination in biological samples.
Target
Glutathione S-Transferase
SampleType
Cell Lysates, Tissues
Detection
Colorimetric (340 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • Glutathione: Powder
  • CDNB
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible)
Conditions
Shipped Ambient, Store at 4°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/18/2024