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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-K336-100 100 tst $635 
Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

Glucose Uptake Assay Kit (Fluorometric) - LS-K336

Glucose Uptake Assay Kit (Fluorometric) - LS-K336

Available for shipment within the USA only
Description:
Glucose uptake has a variety of methods and transporters, and depends upon the metabolic demand of the cell type and availability of glucose. There are over ten different facilitated diffusion glucose transporters which transports glucose down its concentration gradient without ATP hydrolysis. In the kidneys, secondary active transport is used to uptake Glucose against its concentration gradient to ensure that very little glucose is excreted in urine. The fluorescent cell-based glucose uptake assay uses 2- deoxyglucose (2-DG), a widely used glucose analog because it can be taken up by glucose transporters and metabolized by endogenous hexokinase into 2-deoxyglucose 6-phosphate (2-DG6P). 2-DG6P accumulates intracellularly because it is not a suitable substrate for phosphoglucose isomerase, the next step in glycolysis. The cells are lysed, and excess NADP and glucose 6-phosphate dehydrogenase (G6PDH) is added to metabolize 2-DG6P and generate a molar equivalent amount of NADPH. The NADPH is then measured using a G6PDH recycling reaction to amplify the signal and generate a fluorescent signal measureable at gamma ex/em = 530/585 nm proportional to the concentration of 2-DG6P.
Price
Catalog Number
$635 
LS-K336-100
100 tst

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Glucose uptake has a variety of methods and transporters, and depends upon the metabolic demand of the cell type and availability of glucose. There are over ten different facilitated diffusion glucose transporters which transports glucose down its concentration gradient without ATP hydrolysis. In the kidneys, secondary active transport is used to uptake Glucose against its concentration gradient to ensure that very little glucose is excreted in urine. The fluorescent cell-based glucose uptake assay uses 2- deoxyglucose (2-DG), a widely used glucose analog because it can be taken up by glucose transporters and metabolized by endogenous hexokinase into 2-deoxyglucose 6-phosphate (2-DG6P). 2-DG6P accumulates intracellularly because it is not a suitable substrate for phosphoglucose isomerase, the next step in glycolysis. The cells are lysed, and excess NADP and glucose 6-phosphate dehydrogenase (G6PDH) is added to metabolize 2-DG6P and generate a molar equivalent amount of NADPH. The NADPH is then measured using a G6PDH recycling reaction to amplify the signal and generate a fluorescent signal measureable at gamma ex/em = 530/585 nm proportional to the concentration of 2-DG6P.

Specifications

Name
Glucose Uptake Assay Kit (Fluorometric)
Type
Detection/Quantition
Usage
Determination of glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport.
Target
Glucose
SampleType
Cell Cultures
Detection
Fluorometric (Ex/Em 530/585 nm)
Components
  • Assay Buffer
  • G6P Reagent
  • Enzyme A
  • Enzyme B
  • G6PDH Enzyme
  • 2-DG Substrate
  • 2-DG6P Standard
  • NADP
  • Probe
  • NADP/NADPH Extraciton Buffers
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee

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    Customer Reviews (0)

    Images

    Colorimetry

    Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

    Colorimetry

    Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

    Colorimetry

    Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

    Colorimetry

    Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

    Requested From: United States
    Date Requested: 2/24/2020