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Glucose Uptake Assay Kit (Fluorometric) - LS-K336

Available for shipment within the USA only
Catalog
Size
Price
LS-K336-100
100 tst
$620

Available for shipment within the USA only

Product Description

Glucose uptake has a variety of methods and transporters, and depends upon the metabolic demand of the cell type and availability of glucose. There are over ten different facilitated diffusion glucose transporters which transports glucose down its concentration gradient without ATP hydrolysis. In the kidneys, secondary active transport is used to uptake Glucose against its concentration gradient to ensure that very little glucose is excreted in urine. The fluorescent cell-based glucose uptake assay uses 2- deoxyglucose (2-DG), a widely used glucose analog because it can be taken up by glucose transporters and metabolized by endogenous hexokinase into 2-deoxyglucose 6-phosphate (2-DG6P). 2-DG6P accumulates intracellularly because it is not a suitable substrate for phosphoglucose isomerase, the next step in glycolysis. The cells are lysed, and excess NADP and glucose 6-phosphate dehydrogenase (G6PDH) is added to metabolize 2-DG6P and generate a molar equivalent amount of NADPH. The NADPH is then measured using a G6PDH recycling reaction to amplify the signal and generate a fluorescent signal measureable at gamma ex/em = 530/585 nm proportional to the concentration of 2-DG6P.

Specifications

Name
Glucose Uptake Assay Kit (Fluorometric)
Product Type
Detection/Quantition
Usage Summary
Determination of glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport.
Target
Glucose
Sample Type
Cell Cultures
Detection
Fluorometric (Ex/Em 530/585 nm)
Kit Components
  • Assay Buffer
  • G6P Reagent
  • Enzyme A
  • Enzyme B
  • G6PDH Enzyme
  • 2-DG Substrate
  • 2-DG6P Standard
  • NADP
  • Probe
  • NADP/NADPH Extraciton Buffers
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Colorimetry

    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

    Colorimetry

    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

    Colorimetry

    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

    Colorimetry

    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
    Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

    Requested From: United States
    Date Requested: 6/16/2019
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