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Catalog Number Size Price
LS-K336-100 100 Tests $795 
Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

Glucose Uptake Assay Kit (Fluorometric) - LS-K336

Glucose Uptake Assay Kit (Fluorometric) - LS-K336

Available for shipment within the USA only
Description:
Glucose uptake has a variety of methods and transporters, and depends upon the metabolic demand of the cell type and availability of glucose. There are over ten different facilitated diffusion glucose transporters which transports glucose down its concentration gradient without ATP hydrolysis. In the kidneys, secondary active transport is used to uptake Glucose against its concentration gradient to ensure that very little glucose is excreted in urine. The fluorescent cell-based glucose uptake assay uses 2- deoxyglucose (2-DG), a widely used glucose analog because it can be taken up by glucose transporters and metabolized by endogenous hexokinase into 2-deoxyglucose 6-phosphate (2-DG6P). 2-DG6P accumulates intracellularly because it is not a suitable substrate for phosphoglucose isomerase, the next step in glycolysis. The cells are lysed, and excess NADP and glucose 6-phosphate dehydrogenase (G6PDH) is added to metabolize 2-DG6P and generate a molar equivalent amount of NADPH. The NADPH is then measured using a G6PDH recycling reaction to amplify the signal and generate a fluorescent signal measureable at gamma ex/em = 530/585 nm proportional to the concentration of 2-DG6P.
Price
Catalog Number
$795
LS-K336-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
Glucose uptake has a variety of methods and transporters, and depends upon the metabolic demand of the cell type and availability of glucose. There are over ten different facilitated diffusion glucose transporters which transports glucose down its concentration gradient without ATP hydrolysis. In the kidneys, secondary active transport is used to uptake Glucose against its concentration gradient to ensure that very little glucose is excreted in urine. The fluorescent cell-based glucose uptake assay uses 2- deoxyglucose (2-DG), a widely used glucose analog because it can be taken up by glucose transporters and metabolized by endogenous hexokinase into 2-deoxyglucose 6-phosphate (2-DG6P). 2-DG6P accumulates intracellularly because it is not a suitable substrate for phosphoglucose isomerase, the next step in glycolysis. The cells are lysed, and excess NADP and glucose 6-phosphate dehydrogenase (G6PDH) is added to metabolize 2-DG6P and generate a molar equivalent amount of NADPH. The NADPH is then measured using a G6PDH recycling reaction to amplify the signal and generate a fluorescent signal measureable at gamma ex/em = 530/585 nm proportional to the concentration of 2-DG6P.

Specifications

Name
Glucose Uptake Assay Kit (Fluorometric)
Type
Detection/Quantition
Usage
Determination of glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport.
Target
Glucose
SampleType
Cell Cultures
Detection
Fluorometric (Ex/Em 530/585 nm)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • G6P Reagent
  • Enzyme A
  • Enzyme B
  • G6PDH Enzyme
  • 2-DG Substrate
  • 2-DG6P Standard
  • NADP
  • Probe
  • NADP/NADPH Extraciton Buffers
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (fluorometric)
Equipment
Microplate fluorometer
Conditions
Shipped -20°C Dry Ice, Store at -20°C
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Images

Colorimetry

Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

Colorimetry

Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

Colorimetry

Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

Colorimetry

Glucose Assay Kit - Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.
Glucose transport inhibition curve with phloretin. PANC-1 cells were seeded at 10,000 cells per well.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/19/2024