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Glucose Uptake Assay Kit (Colorimetric) - LS-K618

Available for shipment within the USA only
Catalog
Size
Price
LS-K618-100
100 asy
$1,365

Available for shipment within the USA only

Product Description

Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In The glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.

Specifications

Name
Glucose Uptake Assay Kit (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.
Target
Glucose
Sample Type
Adherent Cell Culture, Suspension Cell Culture
Detection
Colorimetric (412 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • Extraction Buffer
  • Neutralization Buffer
  • 2-Deoxyglucose (2-DG
  • 10 mM)
  • Assay Buffer
  • Enzyme Mix (lyophilized)
  • Recycling Mix (lyophilized)
  • 2-DG6P Standard (lyophilized)
  • Glutathione Reductase (lyophilized)
  • Substrate-DTNB (lyophilized)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C for up to 1 year., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.
    2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.

    Assay Result

    2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.
    2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.

    Assay Result

    2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.
    2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.

    Assay Result

    2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.
    2-DG uptake in 3T3-L1, human adipocyte and Hela cells. To scale on the same graph, data from 3T3-L1 cells is plotted at 10% of true value. 2-DG = 2-deoxyglucose, I = Insulin; P = Phloretin.

    Requested From: United States
    Date Requested: 6/16/2019
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