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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Fumarate Assay Kit (Colorimetric) - LS-K136

Available for shipment within the USA only
Catalog
Size
Price
LS-K136-100
100 asy
$500

Available for shipment within the USA only

Product Description

Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb's cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy. LSBio's Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples. The fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (? = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 µM).

Specifications

Name
Fumarate Assay Kit (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection and measurement of Fumarate in various biological samples.
Target
Fumarate
Sample Type
Biological Fluids, Plasma, Serum, Tissue Culture Supernatants, Urine
Detection
Colorimetric (450 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • Fumarate Assay Buffer
  • Fumarate Enzyme Mix
  • Fumarate Developer
  • Fumarate Standard (100 mM)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    Fumarate Assay Kit standard curve.
    Fumarate Assay Kit standard curve.

    Assay Result

    Fumarate Assay Kit standard curve.
    Fumarate Assay Kit standard curve.

    Assay Result

    Fumarate Assay Kit standard curve.
    Fumarate Assay Kit standard curve.

    Assay Result

    Fumarate Assay Kit standard curve.
    Fumarate Assay Kit standard curve.

    Requested From: United States
    Date Requested: 4/23/2019
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