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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

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FLICE/Caspase-8 Assay Kit (Colorimetric) - LS-K8
Available for shipment within the USA only
Catalog
Size
Price
LS-K8-25
25 asy
$250
LS-K8-100
100 asy
$470
LS-K8-200
200 asy
$665
LS-K8-400
400 asy
$1,050

Available for shipment within the USA only

Product Description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.

Specifications

Name
FLICE/Caspase-8 Assay Kit (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.
Target
CASP8 / Caspase 8
Sample Type
Cell Lysates, Tissue Homogenates
Detection
Colorimetric (400/405 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • IETD-pNA (4 mM)
  • DTT (1 M)
  • Dilution Buffer
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible), Cuvette
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About CASP8 / Caspase 8
    Q14790 nm_001228 AAC50645.1

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    Requested From: United States
    Date Requested: 12/13/2018
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