Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-K716-100 100 asy $440 
Collagenase Assay Kit - Inhibition of Collagenase activity by (1,10)-Phenanthroline (IC50 110.5 µM), a Collagenase inhibitor.

Collagenase Inhibitor Screening Kit (Fluorometric) - LS-K716

Collagenase Inhibitor Screening Kit (Fluorometric) - LS-K716

Available for shipment within the USA only
Description:
Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase (MMP) family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria and tumor cell invasion. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren's contracture, an affliction characterized by a thickening of connective tissue. The Collagenase Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing potential inhibitors of Collagenase. The kit uses Self-Quenched BODIPY conjugate of Gelatin (Type B) as a fluorogenic substrate to monitor the activity of Collagenase. Gelatin consists of a heterogeneous mixture of proteins of high average molecular weight derived from collagen Upon proteolytic digestion of the highly quenched BODIPY-labeled gelatin by Collagenase, the de-quenched BODIPY yields bright green fluorescence that can be assayed using a fluorometer or a fluorescence microplate reader (Ex/Em: 490 nm / 520 nm, 515 nm cutoff). In the presence of a Collagenase inhibitor, the gelatin is not digested, the dequenching of BODIPY does not occur and the fluorescent signal is not produced.
Price
Catalog Number
$440 
LS-K716-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase (MMP) family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria and tumor cell invasion. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren's contracture, an affliction characterized by a thickening of connective tissue. The Collagenase Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing potential inhibitors of Collagenase. The kit uses Self-Quenched BODIPY conjugate of Gelatin (Type B) as a fluorogenic substrate to monitor the activity of Collagenase. Gelatin consists of a heterogeneous mixture of proteins of high average molecular weight derived from collagen Upon proteolytic digestion of the highly quenched BODIPY-labeled gelatin by Collagenase, the de-quenched BODIPY yields bright green fluorescence that can be assayed using a fluorometer or a fluorescence microplate reader (Ex/Em: 490 nm / 520 nm, 515 nm cutoff). In the presence of a Collagenase inhibitor, the gelatin is not digested, the dequenching of BODIPY does not occur and the fluorescent signal is not produced.

Specifications

Name
Collagenase Inhibitor Screening Kit (Fluorometric)
Type
Inhibitor Screening
Usage
Screening/studying/characterizing potential Collagenase inhibitors. A simple and quick assay to fluorometrically (Ex/Em = 490/520 nm, 515 nm cut-off) screen/study/characterize potential inhibitors of Collagenase.
Target
Collagenase
Detection
Fluorometric (Ex/Em 490/520 nm)
Components
  • Collagenase Assay Buffer
  • Collagenase Substrate
  • Collagenase
  • Inhibitor (400 mM 1,10-Phenanthroline)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer, Fluorometer
    Conditions
    Shipped -20°C Dry Ice, Components stored at varying temperatures. See recommended storage details in product insert, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Detects using a fluorometer or fluorescence microplate reader
    • Simple and quick protocol

    Publications (0)

    Customer Reviews (0)

    Images

    Assay Result

    Collagenase Assay Kit - Inhibition of Collagenase activity by (1,10)-Phenanthroline (IC50 110.5 µM), a Collagenase inhibitor.
    Inhibition of Collagenase activity by (1,10)-Phenanthroline (IC50 110.5 µM), a Collagenase inhibitor.

    Assay Result

    Collagenase Assay Kit - Inhibition of Collagenase activity by (1,10)-Phenanthroline (IC50 110.5 µM), a Collagenase inhibitor.
    Inhibition of Collagenase activity by (1,10)-Phenanthroline (IC50 110.5 µM), a Collagenase inhibitor.

    Assay Result

    Collagenase Assay Kit - Inhibition of Collagenase activity by (1,10)-Phenanthroline (IC50 110.5 µM), a Collagenase inhibitor.
    Inhibition of Collagenase activity by (1,10)-Phenanthroline (IC50 110.5 µM), a Collagenase inhibitor.

    Assay Result

    Collagenase Assay Kit - Inhibition of Collagenase activity by (1,10)-Phenanthroline (IC50 110.5 µM), a Collagenase inhibitor.
    Inhibition of Collagenase activity by (1,10)-Phenanthroline (IC50 110.5 µM), a Collagenase inhibitor.

    Requested From: United States
    Date Requested: 2/23/2020