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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Viability Assay Kits (Colorimetric) - LS-K238

Available for shipment within the USA only
Catalog
Size
Price
LS-K238-500
500 tst
$170

Available for shipment within the USA only

Product Description

The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Therefore, assays for calculating cell viability are necessary for optimizing cell culture conditions, evaluating cell growth factors and nutrients, discovering novel antibiotics and anti-cancer drugs, evaluating toxic effects of environmental pollutants and cell mediated toxicity and studying programmed cell death (apoptosis). The assay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. This cellular reduction reaction involves the pyridine nucleotide cofactors NADH/NADPH and is only catalyzed by living cells. The formazan product has a low aqueous solubility and is present as purple crystals. Dissolving the resulting formazan with a solubilization buffer permits the convenient quantification of product formation. The intensity of the product color, measured at 550 - 620 nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation.

Specifications

Name
Cell Viability Assay Kits (Colorimetric)
Product Type
Cell Viability
Usage Summary
Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents. As low as 950 cells can be accurately quantified.
Target
Cell Viability
Sample Type
Cell Cultures
Detection
Colorimetric (570 nm Absorbance)
Kit Components
  • Reagent
  • Solubilizer
  • Control Reagent
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped Ambient, Store at Store at 4°C or -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Colorimetry

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    Colorimetry


    Requested From: United States
    Date Requested: 6/19/2019
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