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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cathepsin S Activity Kit (Fluorometric) - LS-K73

Available for shipment within the USA only
Catalog
Size
Price
LS-K73-100
100 asy
$500

Available for shipment within the USA only

Product Description

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-S Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate Z-VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The cathepsin-S assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized to obtain the maximal activity.

Specifications

Name
Cathepsin S Activity Kit (Fluorometric)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Cathepsin-S in various biological sample types. Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
Target
CTSS / Cathepsin S
Sample Type
Cell Lysates, Tissue Homogenates
Detection
Fluorometric (Ex/Em 400/505 nm)
Species Reactivity
Mammal
Kit Components
  • CS Cell Lysis Buffer
  • CS Reaction Buffer
  • CS Substrate Z-VVR-AFC (10 mM)
  • CS Inhibitor (1 mM)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer, Cuvette
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 6-12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About CTSS / Cathepsin S
    P25774 NM_004079 NP_004070.3

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    Requested From: United States
    Date Requested: 5/20/2019
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