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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-K344-100 100 asy $620 
Assay Kit

Caspase-1 Inhibitor Drug Screening Kit (Fluorometric) - LS-K344

Caspase-1 Inhibitor Drug Screening Kit (Fluorometric) - LS-K344

Available for shipment within the USA only
Description:
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-1 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate YVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-1 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Price
Catalog Number
$620 
LS-K344-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-1 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate YVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-1 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.

Specifications

Name
Caspase-1 Inhibitor Drug Screening Kit (Fluorometric)
Type
Inhibitor Screening
Usage
Effective means for screening caspase inhibitors using fluorometric methods.
Target
CASP1 / Caspase 1
Synonyms
CASP1 | Caspase-1 | CASP-1 | CASP1 nirs variant 1 | Caspase 1 | ICE | ICE-like protease | IL-1 beta-converting enzyme | IL-1BC | IL1BC | IL1BCE | p45 | IL1B-convertase | Interleukin-1 beta convertase
Detection
Fluorometric (Ex/Em 400/505 nm)
Species Reactivity
Mammal
Components
  • 2X Reaction Buffer
  • Caspase Substrate YVAD-AFC (1 mM)
  • DTT (1 M)
  • Active Caspase-1 (Lyophilized)
  • Caspase Inhibitor
  • Z-VAD-FMK (2 mM)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles, 6-12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Fast and convenient
    • Inhibition of Caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors
    • Simple one-step procedure
    • Takes only 1-1.5 hours

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    Requested From: United States
    Date Requested: 4/5/2020