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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Bioluminescence Cytotoxicity Assay Kit (Chemiluminescent) - LS-K414

Available for shipment within the USA only
Assay Kit
Catalog
Size
Price
LS-K414-500
500 asy
$325

Available for shipment within the USA only

Product Description

Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Adenylate kinase (AK) is a ubiquitous protein present in all eukaryotic and prokaryotic cells and rapidly release into culture medium upon damage of the plasma membrane. The Bioluminescence Cytotoxicity Assay Kit is based on the measurement of AK in a simple one-step procedure involving two chemical reactions. The first reaction is the conversion of ADP to ATP by adenylate kinase that was released from the damaged cells. The second reaction utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or beta counter. The assay is highly sensitive and can be fully automatic for high throughput.

Specifications

Name
Bioluminescence Cytotoxicity Assay Kit (Chemiluminescent)
Product Type
Cytotoxicity Assay
Target
Cell Viability
Detection
Luminescence
Species Reactivity
Mammal
Kit Components
  • Catalyst
  • Lyophilized
  • Dye Solution
  • AK Detection Reagent (Lyophilized)
  • AK Assay Buffer
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (chemiluminescent)
    Equipment
    Microplate luminometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C for up to 1 year., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Requested From: United States
    Date Requested: 6/19/2019
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