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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Ascorbic Acid (FRASC) Assay Kit II (Colorimetric) - LS-K32

Available for shipment within the USA only
Catalog
Size
Price
LS-K32-100
100 asy
$525

Available for shipment within the USA only

Product Description

Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference. LSBio's FRASC Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Fe+3 is reduced toFe+2 by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.

Specifications

Name
Ascorbic Acid (FRASC) Assay Kit II (Colorimetric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection of 0.2 to 20 nmol Ascorbic Acid (Vitamin C) in various biological sample types.
Target
Vitamin C / Ascorbic Acid
Sample Type
Biological Fluids, Food and Beverages, Growth Medium, Plasma, Serum, Tissue Culture Supernatants, Urine
Detection
Colorimetric (545/600 nm Absorbance)
Species Reactivity
Mammal
Kit Components
  • FRASC Buffer
  • Ascorbic Acid Probe
  • FeCl3 solution
  • Ascorbate Oxidase (lyophilized)
  • Ascorbic Acid Standard (20 -¦mole)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (visible)
    Equipment
    Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Customer Reviews (0)


    Images

    Assay Result

    Ascorbic Acid (Vitamin C) Assay standard curve.
    Ascorbic Acid (Vitamin C) Assay standard curve.

    Assay Result

    Ascorbic Acid (Vitamin C) Assay standard curve.
    Ascorbic Acid (Vitamin C) Assay standard curve.

    Assay Result

    Ascorbic Acid (Vitamin C) Assay standard curve.
    Ascorbic Acid (Vitamin C) Assay standard curve.

    Assay Result

    Ascorbic Acid (Vitamin C) Assay standard curve.
    Ascorbic Acid (Vitamin C) Assay standard curve.

    Requested From: United States
    Date Requested: 7/19/2019
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