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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ApoBrdU DNA Fragmentation Assay Kit (Fluorometric) - LS-K75

Available for shipment within the USA only
Catalog
Size
Price
LS-K75-60
60 asy
$775

Available for shipment within the USA only

Product Description

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. LSBio's Apo-BrdU In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.

Specifications

Name
ApoBrdU DNA Fragmentation Assay Kit (Fluorometric)
Product Type
TUNEL Assay
Usage Summary
The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections. The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.
Target
Apoptosis
Sample Type
Cell Cultures, Tissues
Detection
Fluorometric (Ex/Em 488/520 nm for FITC, and 488/623 nm for PI)
Species Reactivity
Mammal
Kit Components
  • Positive Control Cells
  • Negative Control Cells
  • Wash Buffer-120ml
  • Reaction Buffer
  • TdT Enzymes
  • Br-dUTP
  • Rinse Buffer
  • Anti-BrdU-FITC Antibody
  • PI/RNase Staining Buffer
  • Store at -20° C
  • (See Datasheet for specific volumes supplied)
  • Applications
    Flow Cytometry, Microscopy (fluorescent)
    Equipment
    Flow cytometer, Fluorescence microscope
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Requested From: United States
    Date Requested: 2/18/2019
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