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Catalog Number Size Price
LS-K195-100 100 Tests $530 
Alpha-Mannosidase Assay Kit

Alpha-Mannosidase (AMA) Assay Kit (Colorimetric) - LS-K195

Alpha-Mannosidase (AMA) Assay Kit (Colorimetric) - LS-K195

Available for shipment within the USA only
Description:
Alpha-MANNOSIDASE (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. Alpha-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes Alpha-mannosidosis and leads to deterioration of the central nervous system in children. This non-radioactive, colorimetric AMA assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
Price
Catalog Number
$530
LS-K195-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
Alpha-MANNOSIDASE (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. Alpha-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes Alpha-mannosidosis and leads to deterioration of the central nervous system in children. This non-radioactive, colorimetric AMA assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

Specifications

Name
Alpha-Mannosidase (AMA) Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
For quantitative determination of Alpha-mannosidase enzyme activity using a p-nitrophenol based substrate.
Target
Alpha-Mannosidase
SampleType
Biological Samples
Detection
Colorimetric (405 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Substrate
  • Stop Reagent
  • Standard 12.5 mM Nitrophenol
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible)
Conditions
Shipped Ambient, Store at 4°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

Alpha-Mannosidase Assay Kit

Colorimetry

Alpha-Mannosidase Assay Kit

Colorimetry

Alpha-Mannosidase Assay Kit

Colorimetry

Alpha-Mannosidase Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/15/2024