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Catalog Number Size Price
LS-K167-100 100 Tests $565 
Alpha Amylase Assay Kit

Alpha-Amylase Assay Kit (Colorimetric) - LS-K167

Alpha-Amylase Assay Kit (Colorimetric) - LS-K167

Available for shipment within the USA only
Description:
AMYLASE belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on Alpha-1,4-glycosidic bonds. The Alpha-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, a-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure. Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. This a-amylase assay Method involves two steps: (1). Alpha-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by Alpha-glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen peroxide concentration is determined with a colorimetric reagent.
Price
Catalog Number
$565
LS-K167-100

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
AMYLASE belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on Alpha-1,4-glycosidic bonds. The Alpha-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, a-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure. Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. This a-amylase assay Method involves two steps: (1). Alpha-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by Alpha-glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen peroxide concentration is determined with a colorimetric reagent.

Specifications

Name
Alpha-Amylase Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
For quantitative determination of Alpha-amylase activity.
Target
Alpha Amylase
SampleType
Agricultural Samples, Blood, Saliva, Urine
Detection
Colorimetric (585 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer (pH 7.0)
  • Substrate
  • Detection R eagent
  • Enzyme A
  • Glucose standard Enzyme B
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible)
Conditions
Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

Alpha Amylase Assay Kit

Colorimetry

Alpha Amylase Assay Kit

Colorimetry

Alpha Amylase Assay Kit

Colorimetry

Alpha Amylase Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/15/2024