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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ADP Assay Kit (Colorimetric/Fluorometric) - LS-K36

Available for shipment within the USA only
Catalog
Size
Price
LS-K36-100
100 asy
$575

Available for shipment within the USA only

Product Description

ADP is a product of ATP dephosphorylating and it can be rephosphorylated to ATP. Dehosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. This newly designed ADP Assay Kriot vpides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (OD = 570 nm) or fluorometric methods (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1 µM ADP in biological samples.

Specifications

Name
ADP Assay Kit (Colorimetric/Fluorometric)
Product Type
Detection/Quantition
Usage Summary
This assay provides for the detection of ADP in various biological sample types.
Target
ADP / Adenosine Diphosphate
Sample Type
Biological Fluids, Cell Culture Media, Cell Lysates, Tissue Homogenates
Detection
Colorimetric (570 nm Absorbance), Fluorescent (Ex/Em 535/587 nm)
Species Reactivity
Mammal
Kit Components
  • ADP Assay Buffer
  • ADP Probe (in DMSO)
  • ADP Converter
  • ADP Developer Mix
  • ADP Standard (1 -¦mole lyophilized)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric), Spectrophotometry (visible)
    Equipment
    Microplate fluorometer, Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Images

    Assay Result

    ADP Assay Kit standard curves.
    ADP Assay Kit standard curves.

    Assay Result

    ADP Assay Kit standard curves.
    ADP Assay Kit standard curves.

    Assay Result

    ADP Assay Kit standard curves.
    ADP Assay Kit standard curves.

    Assay Result

    ADP Assay Kit standard curves.
    ADP Assay Kit standard curves.

    Requested From: United States
    Date Requested: 7/19/2019
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