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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Adenylate Kinase (AK) Activity Kit (Color/Fluor) - LS-K37

Available for shipment within the USA only
Catalog
Size
Price
LS-K37-100
100 asy
$525

Available for shipment within the USA only

Product Description

Adenylate Kinase (AK) (EC 2.7.4.3) is an abundant enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. The enzyme is found in the nucleus, cytosol, or mitochondria (intermembrane space or matrix) of various kinds of tissues. Adenylate kinase acts on two molecules of ADP to generate ATP and AMP. Nine isoforms of adenylate kinase have been identified. Erythrocyte adenylate kinase deficiency is associated with hemolytic anemia. Adenylate kinase also plays an important role in post-ischemic recovery and in apoptosis. LSBio's AK Activity Assay kit can kinetically measure Adenylate Kinase activity by detecting adenosine triphosphate (ATP) generated from adenosine diphosphate (ADP) as a substrate. ATP is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the Adenylate Kinase Probe forming an end product that can be measured colorimetrically (OD 570 nm) or fluorometrically (Ex/Em = 535/587 nm).

Specifications

Name
Adenylate Kinase (AK) Activity Kit (Color/Fluor)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Adenylate Kinase (AK) activity in various biological sample types.
Target
Adenylate Kinase
Sample Type
Cell Lysates, Mitochondrial Lysate, Purified Protein, Tissue Homogenates
Detection
Colorimetric (570 nm Absorbance), Fluorescent (Ex/Em 535/587 nm)
Kit Components
  • AK Assay Buffer
  • AK Probe
  • ADP Substrate
  • AK Convertor
  • AK Developer
  • Positive Control (AK Enzyme)
  • ATP Standard (1 -¦mol)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric), Spectrophotometry (visible)
    Equipment
    Microplate fluorometer, Microplate spectrophotometer (visible)
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.

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    Customer Reviews (0)


    Images

    Assay Result

    (a) Fluorometric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (50 ng) and Positive Control (4 µl). (b) Colorimetric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (500 ng) and Positive Control (5 µl).
    (a) Fluorometric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (50 ng) and Positive Control (4 µl). (b) Colorimetric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (500 ng) and Positive Control (5 µl).

    Assay Result

    (a) Fluorometric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (50 ng) and Positive Control (4 µl). (b) Colorimetric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (500 ng) and Positive Control (5 µl).
    (a) Fluorometric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (50 ng) and Positive Control (4 µl). (b) Colorimetric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (500 ng) and Positive Control (5 µl).

    Assay Result

    (a) Fluorometric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (50 ng) and Positive Control (4 µl). (b) Colorimetric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (500 ng) and Positive Control (5 µl).
    (a) Fluorometric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (50 ng) and Positive Control (4 µl). (b) Colorimetric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (500 ng) and Positive Control (5 µl).

    Assay Result

    (a) Fluorometric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (50 ng) and Positive Control (4 µl). (b) Colorimetric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (500 ng) and Positive Control (5 µl).
    (a) Fluorometric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (50 ng) and Positive Control (4 µl). (b) Colorimetric quantitation of Adenylate Kinase Activity in bovine heart mitochondrial lysate (500 ng) and Positive Control (5 µl).

    Requested From: United States
    Date Requested: 3/26/2019
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