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Adenosine Deaminase (ADA) Activity Kit (Fluor) - LS-K92

Available for shipment within the USA only
Catalog
Size
Price
LS-K92-100
100 asy
$450

Available for shipment within the USA only

Product Description

Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation. In LSBio's ADA Activity Assay, inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 µU recombinant Adenosine Deaminase. The range of the assay is from 10-100 µU.

Specifications

Name
Adenosine Deaminase (ADA) Activity Kit (Fluor)
Product Type
Enzymatic Activity Assay
Usage Summary
This assay provides for the detection of Adenosine Deaminase (ADA) activity in various biological sample types.
Target
ADA / Adenosine Deaminase
Sample Type
Cell Lysates, Cell Media, Nuclear Extract, Purified Recombinant Protein, Tissue Homogenates
Detection
Fluorometric (Ex/Em 535/587 nm)
Kit Components
  • ADA Assay Buffer (10x)
  • ADA Convertor
  • ADA Developer
  • ADA Substrate
  • ADA Probe
  • ADA Positive Control
  • Inosine Standard (10 mM)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at Store at -20°C., 12 months shelf life.
    Documents
    Data Sheet Data Sheet    SDS SDS
    Restrictions
    For research use only. Available for shipment within the USA only.
    About ADA / Adenosine Deaminase
    P00813 NM_000022 NP_000013.2

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    Customer Reviews (0)


    Images

    Assay Result

    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).
    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).

    Assay Result

    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).
    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).

    Assay Result

    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).
    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).

    Assay Result

    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).
    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).

    Requested From: United States
    Date Requested: 4/19/2019
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