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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-K92-100 100 asy $480 
ADA / Adenosine Deaminase Assay Kit - (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).

Adenosine Deaminase (ADA) Activity Kit (Fluor) - LS-K92

Adenosine Deaminase (ADA) Activity Kit (Fluor) - LS-K92

Available for shipment within the USA only
Description:
Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation. In LSBio's ADA Activity Assay, inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 µU recombinant Adenosine Deaminase. The range of the assay is from 10-100 µU.
Price
Catalog Number
$480 
LS-K92-100
100 asy

Available for shipment within the USA only
Toll Free North America
866-819-4732

Overview

Description:
Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation. In LSBio's ADA Activity Assay, inosine formed from the breakdown of adenosine is detected via a multi-step reaction, resulting in the formation of an intermediate that reacts with the ADA Probe to generate a fluorescent product. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 10 µU recombinant Adenosine Deaminase. The range of the assay is from 10-100 µU.

Specifications

Name
Adenosine Deaminase (ADA) Activity Kit (Fluor)
Type
Enzymatic Activity Assay
Usage
This assay provides for the detection of Adenosine Deaminase (ADA) activity in various biological sample types.
Target
ADA / Adenosine Deaminase
Synonyms
ADA | Adenosine deaminase | ADA1 | Adenosine aminohydrolase
SampleType
Cell Lysates, Cell Media, Nuclear Extract, Purified Recombinant Protein, Tissue Homogenates
Detection
Fluorometric (Ex/Em 535/587 nm)
Components
  • ADA Assay Buffer (10x)
  • ADA Convertor
  • ADA Developer
  • ADA Substrate
  • ADA Probe
  • ADA Positive Control
  • Inosine Standard (10 mM)
  • (See Datasheet for specific volumes supplied)
  • Applications
    Spectrophotometry (fluorometric)
    Equipment
    Microplate fluorometer
    Conditions
    Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.
    Documents
    Restrictions
    For research use only. Available for shipment within the USA only
    Guarantee
    This Assay Kit carries the LSBio 100% Guarantee
    Product Features
    • Rapid and convenient
    • Measures total activity of Adenosine Deaminase with limit of quantification 10 uU recombinant Adenosine Deaminase
    • Range of the assay: 10-100 uU

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    Images

    Assay Result

    ADA / Adenosine Deaminase Assay Kit - (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).
    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).

    Assay Result

    ADA / Adenosine Deaminase Assay Kit - (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).
    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).

    Assay Result

    ADA / Adenosine Deaminase Assay Kit - (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).
    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).

    Assay Result

    ADA / Adenosine Deaminase Assay Kit - (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).
    (a) Adenosine Deaminase Activity in Jurkat Cell (T-lymphocyte) lysate (105 ng) and Positive Control; BG: Background (b) Adenosine Deaminase specific activity in Jurkat cell lysate (105 ng) and rat brain lysate (2 µg).

    Requested From: United States
    Date Requested: 2/21/2020