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Antibodies > YWHAE / 14-3-3 Epsilon > LS-C817740
 
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Catalog Number Size Price
LS-C817740-100 100 µl $412 
YWHAE / 14-3-3 Epsilon Antibody - Anti-YWHAE rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa. (We are unsure as to the identity of these extra bands.)
YWHAE / 14-3-3 Epsilon Antibody - YWHAE was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C:0.5 mg SH-SY5Y Whole Cell Lysate. 4 uL anti-YWHAE rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-YWHAE rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa.
YWHAE / 14-3-3 Epsilon Antibody - Anti-YWHAE rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa. (We are unsure as to the identity of these extra bands.)
YWHAE / 14-3-3 Epsilon Antibody - YWHAE was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C:0.5 mg SH-SY5Y Whole Cell Lysate. 4 uL anti-YWHAE rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-YWHAE rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa.
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Polyclonal Rabbit anti‑Human YWHAE / 14‑3‑3 Epsilon Antibody (Preservative Free, IF, WB) LS‑C817740

Polyclonal Rabbit anti‑Human YWHAE / 14‑3‑3 Epsilon Antibody (Preservative Free, IF, WB) LS‑C817740

Submit Review LSBio Guarantee
Antibody:
YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C817740-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
14-3-3 Epsilon antibody LS-C817740 is an unconjugated rabbit polyclonal antibody to human 14-3-3 Epsilon (YWHAE). Validated for ELISA, ICC, IF, IP and WB.
Target
Human YWHAE / 14-3-3 Epsilon
Synonyms
YWHAE | 14-3-3E | 14-3-3 epsilon | 14-3-3 protein epsilon | MDCR | MDS | KCIP-1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
Recombinant Human 14-3-3 epsilon / YWHAE Protein
Specificity
Human 14-3-3 Epsilon
Applications
  • ICC (1:300 - 1:10000)
  • Immunofluorescence (1:300 - 1:10000)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
  • ELISA (1:5000 - 1:10000)
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About YWHAE / 14-3-3 Epsilon
P62258 NM_006761 NP_006752.1

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Featured Products

YWHAE / 14-3-3 Epsilon Antibody - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: Mouse Brain Tissue lysate The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 14-3-3 Zeta Recombinant Protein Lane 2.: 14-3-3 Beta Recombinant Protein Lane 3.: 14-3-3 Epsilon Recombinant Protein Lane 4.: 14-3-3 Eta Recombinant Protein Lane 5.: 14-3-3 Gamma Recombinant Protein Lane 6.: 14-3-3 Sigma Recombinant Protein Lane 7.: 14-3-3 Tau Recombinant Protein
YWHAE / 14‑3‑3 Epsilon Antibody (clone AT4F8) LS‑C755593
Species: Human
Applications: ICC, Western blot, Flow Cytometry, ELISA
YWHAE / 14-3-3 Epsilon Protein
Human YWHAE / 14-3-3 Epsilon Protein (Recombinant) - LS-G3598
Source: E. coli
Tag: None
Product
Human YWHAE / 14-3-3 Epsilon Protein (Recombinant GST, N-terminus) (Met1-Gln255) - LS-G39538
Source: E. coli
Tag: GST, N-terminus
Product
Human FASLG / Fas Ligand Protein (Recombinant DDK (Flag)) (aa103-281) - LS-G3868
Source: Human
Tag: DDK (Flag)
NRAS / N-ras Antibody - NRAS Antibody western blot of A431 and rat C6 cell line lysates (35 ug/lane). The NRAS antibody detected the NRAS protein (arrow).
NRAS / N‑ras Antibody (aa70‑101, clone 822CT17.5.1) LS‑C344579
Species: Human, Rat
Applications: Western blot
Alpha Amylase ELISA Kit
Human Alpha Amylase (Sandwich ELISA) ELISA Kit - LS-F4946
Reactivity: Human
Range: 1.56-100 ng/ml
See all YWHAE / 14-3-3 Epsilon Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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