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Catalog Number Size Price
LS-C60090-100 100 µg (1 mg/ml) $425 
Yeast Mer2 Antibody - Anti-Mer2 pS30 Antibody - Western Blot. Western blot of affinity purified anti-S. cerevisiae Mer2 pS30 antibody shows detection of phos-phosphorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Yeast Mer2 Antibody - Anti-Mer2 pS30 Antibody - Western Blot. Western blot of affinity purified anti-S. cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7500 dilution. Secondary antibody was used at 1:5000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Yeast Mer2 Antibody - Anti-Mer2 pS30 Antibody - Western Blot. Western blot of affinity purified anti-S. cerevisiae Mer2 pS30 antibody shows detection of phos-phosphorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
Yeast Mer2 Antibody - Anti-Mer2 pS30 Antibody - Western Blot. Western blot of affinity purified anti-S. cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7500 dilution. Secondary antibody was used at 1:5000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
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Yeast Mer2 Antibody (phospho‑Ser30) LS‑C60090

Yeast Mer2 Antibody (phospho‑Ser30) LS‑C60090

Antibody:
Rabbit Polyclonal to Yeast Yeast Mer2
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C60090-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Yeast Yeast Mer2
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
Yeast Mer2 antibody LS-C60090 is an unconjugated rabbit polyclonal antibody to yeast Yeast Mer2. Validated for ELISA and WB.
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:10000)
  • ELISA (1:5000 - 1:25000)
Immunogen
Synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
Epitope
pSer30
Specificity
the phosphorylated form of Saccharomyces cerevisiae Mer2 protein at the pS30 residue
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to phosphorylated Mer2 protein by western blotting in the appropriate cell lysate or extract. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for Mer2 phosphorylated at the pS30 residue. Preparation of extracts from cells 4hr after initiation of meiosis is suggested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 1/20/2020