Target
Saccharomyces cerevisiae Yeast Mer2
Reactivity
Saccharomyces cerevisiae
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
Applications
- Western blot (1:1000 - 1:10000)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:25000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to phosphorylated Mer2 protein by western blotting in the appropriate cell lysate or extract. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for Mer2 phosphorylated at the pS30 residue. Preparation of extracts from cells 4hr after initiation of meiosis is suggested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee