Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > WFDC2 / HE4 > LS-C756558
 
Print Page  
Catalog Number Size Price
LS-C756558-10 10 µg $318 
LS-C756558-100 100 µg $470 
WFDC2 / HE4 Antibody - IHC analysis of HE4 using anti-HE4 antibody. HE4 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-HE4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
WFDC2 / HE4 Antibody - IHC analysis of HE4 using anti-HE4 antibody. HE4 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-HE4 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
WFDC2 / HE4 Antibody - IHC analysis of HE4 using anti-HE4 antibody. HE4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-HE4 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
WFDC2 / HE4 Antibody - Western blot analysis of HE4 using anti-HE4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human MDA-MB-231 whole cell lysates,Lane 3: human MDA-MB-453 whole cell lysates,Lane 4: rat thymus tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HE4 at approximately 15KD. The expected band size for HE4 is at 13KD.
WFDC2 / HE4 Antibody - IHC analysis of HE4 using anti-HE4 antibody. HE4 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-HE4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
WFDC2 / HE4 Antibody - IHC analysis of HE4 using anti-HE4 antibody. HE4 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-HE4 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
WFDC2 / HE4 Antibody - IHC analysis of HE4 using anti-HE4 antibody. HE4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-HE4 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
WFDC2 / HE4 Antibody - Western blot analysis of HE4 using anti-HE4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human MDA-MB-231 whole cell lysates,Lane 3: human MDA-MB-453 whole cell lysates,Lane 4: rat thymus tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HE4 at approximately 15KD. The expected band size for HE4 is at 13KD.
1 of 4
2 of 4
3 of 4
4 of 4

Polyclonal Rabbit anti‑Human WFDC2 / HE4 Antibody (IHC, WB) LS‑C756558

Polyclonal Rabbit anti‑Human WFDC2 / HE4 Antibody (IHC, WB) LS‑C756558

Submit Review LSBio Guarantee
Antibody:
WFDC2 / HE4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756558-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
WFDC2 / HE4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HE4 antibody LS-C756558 is an unconjugated rabbit polyclonal antibody to HE4 (WFDC2) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Human WFDC2 / HE4
Synonyms
WFDC2 | Epididymis-specific he4 | Epididymal protein 4 | DJ461P17.6 | EDDM4 | HE4 | WAP5
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human HE4 recombinant protein (Position: E31-F124).
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WFDC2 / HE4
Q14508 NM_006103 NP_006094.3

Publications (0)

Submit a Publication for LS-C756558

Customer Reviews (0)

Submit a review for LS-C756558

Featured Products

WFDC2 / HE4 Antibody - IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-WFDC2 mouse monoclonal antibody.
WFDC2 / HE4 Antibody (clone OTI1B9) LS‑C175346
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
WFDC2 / HE4 Antibody - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human WFDC2 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary
WFDC2 / HE4 Antibody (aa29‑43) LS‑C112980
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
WFDC2 / HE4 Antibody - Detection limit for recombinant GST tagged WFDC2 is 0.3 ng/ml as a capture antibody.
WFDC2 / HE4 Antibody (clone 1A23) LS‑C139169
Species: Human
Applications: ELISA
Product
WFDC2 / HE4 Antibody (aa73‑86) LS‑C154785
Species: Human
Applications: Peptide Enzyme-Linked Immunosorbent Assay
WFDC2 / HE4 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ WFDC2 / HE4 Antibody LS‑B12975
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
WFDC2 / HE4 ELISA Kit
Mouse WFDC2 / HE4 (Sandwich ELISA) ELISA Kit - LS-F20177
Reactivity: Mouse
Range: 0.156-10 ng/ml
See all WFDC2 / HE4 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com