Human VTCN1 / B7-H4

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Recombinant Monoclonal

B7H4/2652R

Unconjugated

Protein A/G purified

Unmodified. Also available Azide-free, Carrier-free.

Recombinant human B7-H4 protein fragment. Antigen Molecular Weight: 35 kDa

T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.

• IHC - Paraffin (1 - 2 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Flow Cytometry (1 - 2 µg/10E6 cells)
• ELISA (2 - 4 µg/ml)

Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Store at 2°C to 8°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q7Z7D3 NCBI: NM_024626 NP_078902.2