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Catalog Number Size Price
LS-C662234-10 10 µg $318 
LS-C662234-100 100 µg $470 
VSX2 / CHX10 Antibody - Western blot analysis of CHX10 using anti-CHX10 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse eye ball tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHX10 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHX10 at approximately 43KD. The expected band size for CHX10 is at 39KD.

Polyclonal Rabbit anti‑Human VSX2 / CHX10 Antibody (WB) LS‑C662234

Polyclonal Rabbit anti‑Human VSX2 / CHX10 Antibody (WB) LS‑C662234

Antibody:
VSX2 / CHX10 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662234-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
VSX2 / CHX10 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CHX10 antibody LS-C662234 is an unconjugated rabbit polyclonal antibody to human CHX10 (VSX2). Validated for WB.
Target
Human VSX2 / CHX10
Synonyms
VSX2 | CHX10 | Homeobox protein CHX10 | MCOP2 | MCOPCB3 | RET1 | HOX10 | Visual system homeobox 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human CHX10 recombinant protein (Position: A249-A361). Human CHX10 shares 90.3% amino acid (aa) sequence identity with mouse CHX10.
Specificity
Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About VSX2 / CHX10
P58304 NM_182894 NP_878314.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024