This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G).
This affinity purified antibody is directed against VSV-G protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
This affinity purified antibody has been tested for use in ELISA and by immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluor-escence assay we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200. At higher concentrations, a filamentous background staining may be present.
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.